Anti-asthmatic mechanism of the Huashanshen dripping pill via suppressing contraction of the airway smooth muscle  

作　　者：Yu Liu Ni-Na Cui Xuan-Shuo Liu Peng-Cheng Lin Lorenzo Pecoraro Giuseppe Venturella Shu-Li Man Wen-Yuan Gao 

机构地区：[1]The Innovation Platform for the Development and Construction of Special Project of Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province(2017-ZJ-Y19),College of pharmacy,Qinghai Nationalities University,Xining 810007,China [2]State Key Laboratory of Food Nutrition and Safety,Key Laboratory of Industrial Microbiology,Ministry of Education,Tianjin Key Laboratory of Industry Microbiology,National and Local United Engineering Lab of Metabolic Control Fermentation Technology,China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China [3]Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency,School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China [4]Department of Agricultural,Food and Forest Sciences,University of Palermo,Palermo,Italy

出　　处：《Traditional Medicine Research》2022年第3期19-27,共9页TMR传统医学研究

基　　金：supported by grants 81673647,81673535,81503086 from National Natural Science Foundation of China.

摘　　要：Background:The Huashanshen(HSS)dripping pill has been widely used in asthma for a long time in China.However,the relaxant mechanism of HSS is not well understood.Methods:In this report,high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify the constituents in rat plasma after oral administration of HSS.Ovalbumin-sensitized allergic asthma and isolated trachea were studied for the anti-asthmatic mechanism of HSS.Results:D-anisodamine,L-anisodamine,scopolamine and atropine were detected in the rat plasma containing HSS.It was clear that the HSS inhibited the release of inflammatory mediators,regulated the balance of T-helper 1 and T-helper 2 to reduce the airway inflammation,and relaxed the tracheal smooth muscle by controlling the KCa channel,Ca^(2+)influx and release to reduce the airway hyperresponsiveness.Conclusion:Atropine,anisodamine and scopolamine might be active compounds of HSS which inhibited the release of inflammatory mediators,regulated the balance of Th1/Th2,and relaxed the tracheal smooth muscle to reduce airway hyperresponsiveness.

关 键 词：Huashanshen dripping pill HPLC-QTOF-MS allergic asthma airway remodeling INFLAMMATION airway smooth muscle 

分 类 号：R56[医药卫生—呼吸系统]