非变性Ⅰ型胶原蛋白对免疫低下大鼠免疫功能的调节作用  

Immune-regulatory Effect of Non-denatured type-Ⅰ Collagen on the Immune Function in Immunocompromised Rats

作　　者：谢金莉 杜丽晶 王超跃 王乐琪 叶春婷 严诗楷[3] 肖雪 李莎莎[2] XIE Jinli;DU Lijing;WANG Chaoyue;WANG Leqi;YE Chunting;YAN Shikai;XIAO Xue;LI Shasha(Guangzhou Trauer Biotechnology Co.Ltd.,Guangzhou 510663,China;The Second Clinical Collage,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Schoolof Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China;Chinese Medicine Research Institute,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广州创尔生物技术股份有限公司,广东广州510663 [2]广州中医药大学第二临床医学院,广东广州510006 [3]上海交通大学药学院,上海200240 [4]广东药科大学中医药研究院,广东广州510006

出　　处：《现代食品科技》2022年第6期66-73,共8页Modern Food Science and Technology

基　　金：广州创尔生物技术股份有限公司科研攻关项目(RD202006)。

摘　　要：该研究旨在观察非变性Ⅰ型胶原蛋白(Non-denatured type-Ⅰ collagen,NDC-Ⅰ)对免疫低下模型大鼠免疫功能的调节作用。动物实验采用腹腔注射环磷酰胺法建立免疫低下大鼠模型,观察NDC-Ⅰ对大鼠的胸腺指数和脾脏指数,白细胞计数和分类,血清中免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、白介素-2(IL-2)、白介素-4(IL-4)、白介素-10(IL-10)、干扰素-γ(IFN-γ)和肿瘤坏死因子α(TNF-α),脾脏和胸腺的乳酸脱氢酶(LDH)活性和组织形态学变化。结果显示,在给予NDC-Ⅰ干预30 d后,与模型组比较,NDC-Ⅰ组胸腺指数上升(p<0.05),白细胞计数显著提高(p<0.01),血清中IgA、IgG、IL-2、IL-4、IL-10、IFN-γ和TNF-α含量为43.32μg/mL、283.32μg/mL、1827.17 ng/L、135.97 pg/mL、113.87 ng/L、2302.44 pg/mL、469.91 ng/L,水平显著升高(p<0.01),脾脏中LDH活性显著上升(p<0.01),脾脏和胸腺组织病理学损伤减轻。结果证明,NDC-Ⅰ对环磷酰胺致免疫低下大鼠的免疫功能具有一定的调节作用,也为胶原蛋白等相关功能产品的综合开发与充分利用提供了参考依据。To investigate the regulatoryeffect of non-denatured type-Ⅰ collagen(NDC-Ⅰ) on the immune function inimmunocompromised rats, the immunocompromised model was established by intraperitoneal injection of cyclophosphamide in animal experiments. The thymus index,spleen index, white blood cell countand classification, IgA(immunoglobulin A) level, IgG(immunoglobulin G) level, IL-2(interleukin-2)level, IL-4(interleukin-4) level, IL-10(interleukin-10) level, IFN-γ(interferon-gamma) level, TNF-α(tumor necrosis factor-α) level, activity of lactate dehydrogenase(LDH) in spleen and thymus, as well as histomorphological changes of spleen and thymus, were examined. The results showed compared with the model group, a 30-day NDC-Ⅰ intervention led to significant increases inthe thymus index(p<0.05) and white blood cell count(p<0.01) of the NDC-Ⅰ treated group, causingsignificant increases(p<0.01) in their levels of serum IgA, IgG, IL-2, IL-4, IL-10, IFN-γ and TNF-α(as 43.32 μg/mL, 283.32 μg/mL, 1827.17 ng/L, 135.97 pg/mL, 113.87 ng/L, 2302.44 pg/mL and 469.91 ng/L, respectively), and the activity of LDH in spleen. In addition, the histopathological damages of the spleen and thymus werereduced. The obtained results revealed that the NDC-Ⅰ treatment showed a regulatory effect on the immune function of cyclophosphamide-induced immunocompromised rats. This research also provides a reference for the comprehensive development and full utilization of collagen and other related functional products.

关 键 词：非变性Ⅰ型胶原蛋白 免疫调节 环磷酰胺 

分 类 号：TS201.4[轻工技术与工程—食品科学]