抗肿瘤多肽M1-21的剂型优化  

Desolution Optimization of Anti-tumor Peptide M1-21

作　　者：谭拥军[1] 裴超柱 程浩杰 卜会铜 谭桂湘[1] 邓磊 TAN Yongjun;PEI Chaozhu;CHENG Haojie;BU Huitong;TAN Guixiang;DENG Lei(College of Biology,Hunan University,Changsha 410082,China)

机构地区：[1]湖南大学生物学院,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2022年第6期146-151,共6页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(81472718)。

摘　　要：通过去溶剂化方法,将单体抗肿瘤多肽M1-21纳米化,形成50 nm左右大小的多肽纳米颗粒(Nano-M1-21).运用动态光色散技术(DLS)和低压透射电镜(TEM)对Nano-M1-21进行表征,并进行体外37℃条件下颗粒稳定性测试.运用乳腺癌MDA-MB-231、MCF-7和4T1细胞验证Nano-M1-21的抗肿瘤效果,结果表明Nano-M1-21更容易被细胞摄取;相比单体多肽,达到抑制肿瘤细胞的浓度更低.运用小鼠乳腺癌4T1细胞活体肿瘤移植模型,发现Nano-M1-21展示出良好的抑瘤效果.总之,单体肽M1-21经纳米剂型优化后,有利于改善细胞的摄取,显示出更好的抑瘤效果.The anti-tumor peptide M1-21 monomer was used to create nanoparticles(Nano-M1-21,about 50nm)by the desolution method. The characteristics of Nano-M1-21 were determined by the dynamic optical dispersion(DLS)and the low pressure transmission electron microscopy(TEM). The stability of Nano-M1-21 particles was measured in vitro at 37 degrees centigrade. To verify the anti-tumor effects of Nano-M1-21,we used breast cancer MDA-MB-231,MCF-7 and 4T1 cells to show that Nano-M1-21 was easily absorbed by the cells and its tumor inhibitory concentration was lower than that of monomer peptide. With a mouse breast cancer 4T1 cell-grafting tumor model,we found that Nano-M1-21 showed a good suppressive effect on the tumors. In conclusion,compared to the monomer peptide M1-21,the optimized Nano-M1-21 nanoparticles provide benefits to improve the cellular uptake and anti-tumor effects.

关 键 词：M1-21 Nano-M1-21 纳米化 抑瘤效果 多肽 

分 类 号：Q784[生物学—分子生物学]