Characterization of laccase gene StLAC6 and its involvement in the pathogenicity and peroxisome function in Setosphaeria turcica  

作　　者：LIU Ning ZHANG Qian-qian JIA Hui ZHAO Bin ZHU Zi-ping CAO Zhi-yan DONG Jin-gao 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,Baoding 071001,P.R.China [2]College of Plant Protection,Hebei Agricultural University,Baoding 071001,P.R.China [3]College of Life Sciences,Hebei Agricultural University,Baoding 071001,P.R.China [4]Langfang Academy of Agriculture and Forestry Science,Langfang 065000,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第7期2019-2030,共12页农业科学学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(31901827);the China Agriculture Research System(CARS-02);the Natural Science Foundation of Hebei Province,China(C2020204039);the Key Research and Development Projects of Hebei Province(20326510D);the Hebei Province Projects of Overseas Foundation(C20190508)。

摘　　要：Laccases, as a kind of multicopper oxidase, play an important role in pigment synthesis and growth in fungi and are involved in their interactions with host plants. In Setosphaeria turcica, 9 laccase-like multicopper oxidases have been identified, and StLAC2 is involved in the synthesis of the melanin that accumulates in the cell wall. The function of another major laccase gene, StLAC6, was studied here. The knockout of StLAC6 had no effect on the growth, morphology or invasion ability of S. turcica, but the morphology and function of peroxisomes of knockout mutants were abnormal. The knockout of the StLAC6 gene resulted in increased contents of phenolic compounds and melanin and the sensitivity to fungicides increased compared with wild type strains. In the mutants of StLAC6, there is a significant change of the expression levels of other laccase genes. This study provides a new insight into laccase functions and the relationship of the laccase gene family in plant pathogenic fungi.

关 键 词：Setosphaeria turcica LACCASE StLAC6 PEROXISOME fungicides 

分 类 号：S432.4[农业科学—植物病理学]