血清Midkine对卵巢癌的诊断价值及其与临床病理特征相关性  

Diagnostic value of serum midkine for ovarian cancers and its correlation with clinicopathological features

作　　者：闵晶 沈国松 张闻雯 MIN Jing;SHEN Guo-song;ZHANG Wen-wen(Huzhou Maternity and Child Health Care Hospital,Huzhou,Zhejiang 313000,China)

机构地区：[1]湖州市妇幼保健院,浙江湖州313000

出　　处：《中国卫生检验杂志》2022年第9期1095-1098,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨血清Midkine(MDK)水平对卵巢癌的诊断及与临床病理特征相关性。方法通过Oncomine数据库和Kaplan-Meier plotter在线数据库,分析卵巢癌组织中MDK的mRNA表达及其与卵巢癌患者预后的关系。同时采用ELISA方法对63例卵巢癌患者,62例良性卵巢癌肿瘤患者和61例健康体检者的血清MDK水平,采用免疫化学发光法检测血清人附睾蛋白4(HE4)水平,应用受试者工作特征曲线(ROC)评价对卵巢癌诊断价值,同时结合卵巢癌患者临床特征分析。结果Oncomine数据库分析显示卵巢癌患者癌组织中MDK的mRNA表达水平显著高于正常组织(P<0.05),Kaplan-Meier plotter分析表明组织中MDK的mRNA高表达患者的总生存期(OS)和无进展生存期(PFS)均高于低表达患者(P<0.05)。卵巢癌患者血清MDK、HE4水平显著高于良性卵巢肿瘤患者和健康对照组(P<0.05)。有淋巴结转移和有周围浸润的卵巢癌患者血清MDK水平显著增高(P<0.05)。MDK对卵巢癌诊断的ROC曲线下面积(AUC)为0.930,灵敏度为81.0%,特异度为95.1%。血清HE4对卵巢癌诊断AUC为0.853,灵敏度和特异度分别为69.8%和98.4%。两者联合诊断卵巢癌的AUC为0.971,灵敏度达到90.5%。结论血清MDK对卵巢癌具有诊断价值,联合HE4可以提高诊断的灵敏度,其还有望成为卵巢癌预后判断的标志物。Objective To investigate the serum Midkine(MDK)level in the diagnosis of ovarian cancer and the correlation with clinicopathological characteristics.Methods The expression of MDK mRNA in ovarian cancer tissues and its relationship with prognosis were analyzed by Oncomine database and Kaplan-Meier plotter online database.The serum MDK levels of 63 patients with ovarian cancer,62 patients with benign ovarian cancer and 61 healthy subjects were detected by immunochemiluminescence.The diagnostic value of ovarian cancer was evaluated by receiver operating characteristic curve(ROC),and the clinical characteristics of ovarian cancer patients were analyzed.Results Oncomine database analysis showed that the mRNA expression of MDK in cancer tissues of ovarian cancer patients was significantly higher than that in normal tissues(P<0.05),and Kaplan-Meier plotter analysis indicated that the high expression of MDK in the tissues of the patients had a higher overall survival(OS)and progression free survival(PFS)than that in the low expression group(P<0.05).The serum levels of MDK and HE4 in patients with ovarian cancer were significantly higher than the latter two(P<0.05).The serum MDK levels in ovarian cancer patients with different tumor infiltration and lymph node metastasis were higher(P<0.05).The area under the ROC curve(AUC)of MDK for ovarian cancer diagnosis was 0.930,with the sensitivity of 81.0%and the specificity of 95.1%.HE4 had a sensitivity of 69.8%and a specificity of 98.4%for ovarian cancer diagnosis,respectively.The AUC of the two biomarkers combined to diagnose ovarian cancer was 0.971,with the sensitivity of 90.5%.Conclusion Serum MDK levels have diagnostic value for ovarian cancer,and combined HE4 can improve the sensitivity.Serum MDK may also be a potential prognostic marker for ovarian cancer.

关 键 词：MIDKINE 卵巢癌 诊断 病理 

分 类 号：R737.31[医药卫生—肿瘤]