DADS抑制XIAP介导的人胃癌HGC27细胞增殖、迁移和侵袭  

DADS inhibits XIAP-mediated proliferation, migration and invasion of human gastric cancer HGC27 cells

作　　者：刘芳 李志敏 曹振华 何慧 苏琦 苏波 LIU Fang;LI Zhimin;CAO Zhenhua;HE Hui;SU Qi;SU Bo(Institute of Cancer,School of Pharmacy,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China;Institute of Applied Anatomy and Reproductive Medicine,School of Basic Medicine,School of Pharmacy,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China;Institute of Pharmacy and Pharmacology,School of Pharmacy,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学衡阳医学院肿瘤研究所,湖南省衡阳市421001 [2]南华大学衡阳医学院基础医学院应用解剖与生殖医学研究所,湖南省衡阳市421001 [3]南华大学衡阳医学院药学院药物药理研究所,湖南省衡阳市421001

出　　处：《中南医学科学杂志》2022年第3期323-326,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81973532);湖南省自然科学基金(2020JJ4522,2020JJ4529);湖南省高校创新平台开放基金(17K081)。

摘　　要：目的 研究二烯丙基二硫(DADS)对X连锁凋亡抑制蛋白(XIAP)过表达胃癌HGC27细胞增殖、迁移和侵袭能力的影响。方法 建立XIAP过表达HGC27细胞株;实验设置为Vector组、Vector+DADS组、XIAP组和XIAP+DADS组;DADS处理细胞后,Western blotting检测各组XIAP;MTT和平板克隆实验分析各组细胞的增殖能力;Transwell实验检测各组细胞的迁移、侵袭能力。结果 XIAP过表达细胞增殖率和克隆形成率增高,迁移和侵袭的细胞数增加;DADS处理XIAP过表达细胞后,XIAP表达降低的同时,细胞增殖率和克隆形成率下降,迁移和侵袭的细胞数减少。结论 过表达XIAP促进HGC27细胞增殖和迁移侵袭;DADS下调XIAP,抑制HGC27细胞增殖、迁移和侵袭。Aim To investigate the effects of diallyl disulfide(DADS) on the proliferation, migration and invasion ability of X-linked inhibitor of apoptosis protein(XIAP) overexpressed gastric cancer cells. Methods XIAP overexpression HGC27 cell line was established. The experiment was set as vector group, vector+DADS group, XIAP group, XIAP+DADS group. After the cells were treated with DADS, Western blotting was used to detect XIAP, MTT and plate cloning assay were used to analyze the proliferation ability of cells, and Transwell assay was used to detect the migration and invasion ability of cells. Results The proliferation rate and clone formation rate increased in XIAP overexpressing cells, meanwhile the number of migrating and invading cells increased. After XIAP overexpressing cells were treated with DADS, XIAP expression decreased, cell proliferation rate and clone formation rate decreased, and the number of migrating and invasive cells decreased. Conclusion Overexpression of XIAP promoted the proliferation, migration and invasion of HGC27 cells, and DADS downregulated XIAP and inhibited HGC27 cell proliferation, migration and invasion.

关 键 词：二烯丙基二硫 X连锁凋亡抑制蛋白 增殖 迁移 侵袭 HGC27细胞 

分 类 号：R735.2[医药卫生—肿瘤]