金雀异黄酮通过调控PI3K/AKT信号通路抑制肺癌细胞增殖和侵袭转移  

Genistein inhibits lung cancer cell proliferation,invasion and metastasis by regulating PI3K/AKT signaling pathway

作　　者：谷田露 王芳[2] 毛琳 王瑶[3] 李爽 GU Tian-lu;WANG Fang;MAO Lin;WANG Yao;LI Shuang(Department of Oncology,Tieling Central Hospital,Tieling 112001;Department of Endocrinology,Tieling Central Hospital,Tieling 112001;Department of Neurology,Tieling Central Hospital,Tieling 112001;Department of Oncology,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110033,China)

机构地区：[1]铁岭市中心医院肿瘤内科,辽宁铁岭112001 [2]铁岭市中心医院内分泌科,辽宁铁岭112001 [3]铁岭市中心医院神经内科,辽宁铁岭112001 [4]辽宁中医药大学附属医院肿瘤科,辽宁沈阳110033

出　　处：《解剖科学进展》2022年第1期63-65,69,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(20180550404)。

摘　　要：目的探讨金雀异黄酮对人肺癌细胞A549的增殖、侵袭和转移的影响及可能机制。方法体外培养人肺癌细胞A549细胞后,将细胞分为对照组、金雀异黄酮10、20、40、80μmol/L组。CCK-8方法检测A549细胞的增殖情况;划痕实验检测肺癌细胞迁移能力;穿梭小室实验检测肺癌细胞侵袭能力的变化;Western blot方法检测各组细胞PI3K/AKT信号通路相关蛋白的表达情况。结果金雀异黄酮能抑制A549细胞的增殖,并呈浓度依赖性和时间依赖性,40μmol/L金雀异黄酮的抑制效果最好。划痕实验和穿梭小室实验表明金雀异黄酮能显著降低A549细胞迁移和侵袭能力,显著降低细胞p-PI3K和p-AKT的表达,但PI3K和AKT的水平无显著变化。结论金雀异黄酮抑制人肺癌细胞A549细胞增殖、迁移和侵袭,其作用机制可能与抑制PI3K和AKT蛋白的磷酸化有关。Objective To investigate the effect and possible mechanism of genistein on proliferation,invasion and migration of human lung cancer cell A549.Methods After cultured in vitro,human lung cancer cell A549 cells were divided into control group and genistein groups of 10,20,40 and 80μmol/L.The proliferation of A549 cells was detected by CCK-8 method.The migration ability of lung cancer cells was detected by scratch test.Transwell test was used to detect the change of invasion ability of lung cancer cells.Western blot was used to detect the expression of PI3 K/AKT signaling pathway related proteins in each group.Results Genistein could inhibit the proliferation of A549 cells in a concentrationdependent and time-dependent manner,and 40μmol/L genistein had the best inhibitory effect.Scratch test and tanswell test showed that the genistein could significantly reduce the migration and invasion ability of A549 cells,and significantly reduce the expression of p-PI3K and p-Akt,but there was no significant change in PI3 K and AKT.Conclusion Genistein inhibits the proliferation,migration and invasion of human lung cancer cell A549 cells,and its mechanism may be related to the inhibition of the phosphorylation of PI3K and AKT.

关 键 词：金雀异黄酮 肺癌 迁移 侵袭 磷脂酰肌醇3-激酶 蛋白激酶B 

分 类 号：R734.2[医药卫生—肿瘤]