秦岭石蝴蝶叶片离体组织培养技术  

In Vitro Tissue Culture Technology of Leaves of Petrocosmea qinlingensis

作　　者：胡选萍[1,2,3] 蒋景龙[1,2,3] 王琦 李耘[4] HU Xuanping;JIANG Jinglong;WANG Qi;LI Yun(College of Biology Sciences and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000;Shaanxi Province Key Laboratory of Bio-resources,Hanzhong,Shaanxi 723000;Qinba State Key Laboratory of Biological Resources and Ecological Environment,Shaanxi University of Fechnology,Hanzhong,Shaanxi 723000;Hanzhong City Wildlife Conservation and Management Station,Hanzhong,Shaanxi 723000)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]陕西省资源生物重点实验室,陕西汉中723000 [3]陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室(培育),陕西汉中723000 [4]汉中市野生动植物保护管理站,陕西汉中723000

出　　处：《北方园艺》2022年第10期70-76,共7页Northern Horticulture

基　　金：汉中市野生动植物保护管理站资助项目(208021840);陕西省科技厅重点研发计划资助项目(2020NY-063)。

摘　　要：以秦岭石蝴蝶(Petrocosmea qinlingensis)幼嫩叶片为试材,采用离体组织培养方法,研究了外植体消毒、初代培养、愈伤组织继代分化、不定芽增殖、生根炼苗对秦岭石蝴蝶离体再生的影响,以期为建立秦岭石蝴蝶组织培养快繁体系提供参考依据。结果表明:0.1%氯化汞处理2min+无菌水洗涤7~8次为最适灭菌方法;最适愈伤组织诱导与继代培养基为MS+2.0mg·L^(-1)IBA;愈伤组织分化的合适培养基为MS+0.1mg·L^(-1)6-BA+0.2mg·L^(-1)NAA+0.1mg·L^(-1)IBA;正交实验结果表明MS+0.1mg·L^(-1)6-BA+0.1mg·L^(-1)IBA+0.1mg·L^(-1)ZT为最适增殖培养基,最大增殖系数达14.06;最适生根炼苗条件为珍珠岩、蛭石和泥炭(2:1:1)基质+MS营养液1mL+200mg·L^(-1)IBA 0.5mL。Taking leaves of Petrocosmea qinlingensis as test materials,the effects of explants disinfection,primary culture,subculture and differentiation of callus,adventitive bud multiplication,rooting and seedling training on regeneration of Petrocosmeaqinlingensis were studied by using in vitro tissue culture method,in order to provide reference for the establishment of rapid propagation system of Petrocosmea qinlingensis.The results showed that optimal disinfection method was treatment with 0.1%mercuric chloride 2 minutes and wash with sterile water 7—8times.Best medium for callus induction and subculture was MS+2.0mg·L^(-1) IBA.Suitable medium for callus differentiation was MS+0.1mg·L^(-1)6-BA+0.2mg·L^(-1) NAA+0.1mg·L^(-1)IBA.The results of orthogonal experiment indicated that the proper condition for adventitious buds induction was MS+0.1mg·L^(-1)6-BA+0.1mg·L^(-1) IBA+0.1mg·L^(-1) ZT and maximum multiplication coefficient up to 14.06.Optimum rooting and growing cultivation conditions was substrate of perlite,vermiculite and peat(2:1:1)+MS nutrient solution(1mL)+200mg·L^(-1) IBA(0.5mL).

关 键 词：秦岭石蝴蝶 叶片 分化 增殖 植株再生 

分 类 号：S567.239[农业科学—中草药栽培]