单细胞转录组测序在眼科领域的应用进展  

Application of single-cell transcriptome sequencing in the field of ophthalmology

作　　者：尹贻雪 焦路光 王嘉睿 郑红[2] 杨在富 Yin Yixue;Jiao Luguang;Wang Jiarui;Zheng Hong;Yang Zaifu(Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100039,China;Basic Medicine College,Anhui Medical University,Hefei 230032,China)

机构地区：[1]军事科学院军事医学研究院辐射医学研究所,北京100039 [2]安徽医科大学基础医学院,合肥230032

出　　处：《国际眼科纵览》2022年第2期184-188,共5页International Review of Ophthalmology

基　　金：国家自然科学基金(61575221)。

摘　　要：单细胞转录组测序通常称为单细胞RNA测序(scRNA-seq),是一种在单个细胞水平上测量基因表达,对其含有的转录组信息进行测序分析的方法。近年来该技术在眼科领域的应用逐渐增加,可用于视网膜或角膜细胞类型与细胞亚型的鉴定;用于研究人类视网膜的早期发育过程、细胞分化的时间进程、视网膜和视网膜类器官的形成过程。该技术也可用于眼损伤修复的分子机制研究,有助于发现年龄相关性黄斑变性、视网膜母细胞瘤等眼部疾病新的生物靶点和标记物,为其治疗提供新的方向。(国际眼科纵览,2022,46:184-188)Single-cell transcriptome sequencing,often referred to single-cell RNA sequencing(scRNA-seq),is a technique to measure gene expression and analyze the transcriptome sequence at the single-cell level.In recent years,the application of the scRNA-seq technique has been gradually increased,especially in the field of ophthalmology.It can be used to identify cell types and cell subtypes of retina and cornea,facilitate the investigation of the early human retina development,the time course of cell differentiation,and the retinal and retinal organoids formation.In addition,single-cell transcriptome sequencing can be used to study molecular mechanisms of wound healing in the eye and help to found new biological targets and markers of eye diseases,such as age-related macular degeneration and retinoblastoma,providing a new direction for the treatment.(Int Rev Ophthalmol,2022,46:184-188)

关 键 词：单细胞转录组测序 单细胞分析 眼科 

分 类 号：R77[医药卫生—眼科]