当归补血汤对2型糖尿病大鼠IRS-1/PI3K/Akt2信号通路的影响研究  

Effect of Danggui Buxue Decoction on the expression of IRS-1,PI3K,Akt2 proteins and their substrates in the rats with type 2 diabetes

作　　者：尹世伟[1] 崔玉兰 贾小玉 赵婷 崔宇晖 康丽艳 YIN Shiwei;CUI Yulan;JIA Xiaoyu;ZHAO Ting;CUI Yuhui;KANG Liyan(The First Hospital of Handan,Handan 056000,Hebei,China;The Eighth People’s Hospital of Hebei Province,Shijiazhuang 050000,Hebei,China;The Second Hospital of Handan,Handan 056000,Hebei,China;Wen’an County Hospital,Langfang 065800,Hebei,China)

机构地区：[1]邯郸市第一医院,河北邯郸056000 [2]河北省第八人民医院,河北石家庄050000 [3]邯郸市第二医院,河北邯郸056000 [4]文安县医院,河北文安065800

出　　处：《现代中西医结合杂志》2022年第10期1369-1374,1395,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：邯郸市科学技术研究与发展计划项目(21422083067)。

摘　　要：目的探讨当归补血汤对2型糖尿病大鼠胰岛素受体底物1(IRS-1)、磷脂酰肌醇3激酶p85亚基(PI3Kp85)、蛋白激酶B(Akt2)表达的影响。方法取8只雄性ZDF(fa/+)大鼠作为对照组;另取24只雄性ZDF(fa/fa)大鼠给予3周高脂饲料建立2型糖尿病模型,然后将造模成功大鼠随机分为模型组、盐酸二甲双胍组、当归补血汤组,每组8只。盐酸二甲双胍组给予盐酸二甲双胍肠溶片300 mg/kg灌胃,当归补血汤组给予当归补血汤(配方颗粒加蒸馏水配制)12.8 g/kg灌胃,对照组和模型组给予等量生理盐水灌胃,均1次/d,连续8周。末次灌胃后,禁食14 h进行口服葡萄糖耐量试验,计算胰岛素生成指数、胰岛素抵抗指数(HOMA-IR)和胰岛β细胞功能指数(HOMA-β);糖耐量试验结束后隔天禁食12 h抽取腹主动脉血,检测丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平;取各组大鼠肝脏组织,采用RT-qPCR法检测IRS-1、PI3Kp85和Akt2 mRNA表达情况,采用Western blot法检测IRS-1、p-IRS1、PI3Kp85、p-PI3Kp85、Akt2、p-Akt2蛋白表达情况。结果模型组大鼠空腹血糖水平、空腹胰岛素水平、血糖曲线下面积、HOMA-IR均明显高于对照组(P均<0.05),胰岛素生成指数、HOMA-β均明显低于对照组(P均<0.05);盐酸二甲双胍组和当归补血汤组空腹血糖水平、空腹胰岛素水平、血糖曲线下面积、HOMA-IR均明显低于模型组(P均<0.05),胰岛素生成指数、HOMA-β均明显高于模型组(P均<0.05),当归补血汤组各指标改善情况均明显优于二甲双胍组(P均<0.05)。与对照组比较,模型组大鼠血清ALT、AST、TC、TG、LDL-C水平均明显升高(P均<0.05),HDL-C水平明显降低(P<0.05);与模型组比较,盐酸二甲双胍组和当归补血汤组大鼠血清ALT、AST、TC、TG、LDL-C水平均明显降低(P均<0.05),HDL-C水平均明显降低(P均<0.05);除ALT、TC外,其余�Objective It is to explore the effects of Danggui Buxue Decoction on the expression of insulin receptor substrate 1(IRS-1),phosphatidylinositol 3-kinase p85 subunit(PI3Kp85),protein kinase B(Akt2)proteins and their substrates in type 2 diabetic rats.Methods Thirty-two male rats were divided into 4 groups.8 male ZDF(fa/+)rats were set as the control group,and 24 male ZDF(fa/fa)rats were fed with high-fat diet for 3 weeks to establish type 2 diabetic models,and were randomly divided into the model group,metformin hydrochloride group and Danggui Buxue Decoction group,8 rats in each group.The metformin hydrochloride group was given metformin hydrochloride enteric-coated tablets 300 mg/kg by gavage,the Danggui Buxue Decoction group was given Danggui Buxue Decoction(prepared with granules and distilled water)12.8 g/kg by gavage,and the control group and model group were given the same amount of normal saline by gavage,all once per day,continuously treated for 8 weeks.After the last gavage,fasting for 14 hours,oral glucose tolerance test was performed to calculate insulin production index,insulin resistance index(HOMA-IR)and isletβcell function index(HOMA-β);fasting for 12 hours on the next day after the glucose tolerance test,abdominal aortic blood was drawn to detect the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triacylglycerol(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C).The liver tissue of the rats in each group was taken to detect the mRNA expression of IRS-1,PI3Kp85 and Akt2 by RT-qPCR method;the pancreas tissue of the rats in each group was collected to detect the expression of IRS-1,p-IRS1,PI3Kp85 and p-PI3Kp85,Akt2,p-Akt2 protein by Western blot method.Results The levels of fasting blood glucose and fasting insulin,area under curve of blood glucose and HOMA-IR of the rats in the model group were significantly higher than those in the control group(all P<0.05),while the insulin production index and HOMA-βwere

关 键 词：当归补血汤 2型糖尿病 大鼠 胰岛素受体底物1 磷脂酰肌醇3激酶p85亚基 蛋白激酶B 

分 类 号：R-332[医药卫生]