Refinement of four major QTL for oil content in Brassica napus by integration of genome resequencing and transcriptomics  

作　　者：Shuxiang Yan Huaixin Li Hongbo Chao Jianjie He Yiran Ding Weiguo Zhao Kai Zhang Yiyi Xiong Kang Chen Libin Zhang Maoteng Li 

机构地区：[1]Department of Biotechnology,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,Hubei,China [2]Key Laboratory of Molecular Biophysics of the Ministry of Education,Wuhan 430074,Hubei,China [3]School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450001,Henan,China

出　　处：《The Crop Journal》2022年第3期627-637,共11页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(31871656 and 32072098)。

摘　　要：Rapeseed(Brassica napus)supplies about half of the vegetable oil in China.Increasing oil production and searching for genes that control oil content in the crop are research goals.In our previous studies,four major QTL for oil content located on A08,A09,C03 and C06 in the Ken C-8×N53-2(KN DH)mapping population were detected.The parental lines were resequenced to identify structural variations and candidate genes affecting oil content in these four major QTL regions.Insertion-deletion(In Del)markers were developed and used to narrow the regions.Differentially expressed genes located in the regions were investigated.GO and KEGG analysis showed that several genes were associated with lipid metabolism.Several transcription factors with higher expression in N53-2 than in Ken C-8 were identified.These results shed light on the genetic control of oil content and may be helpful for the development of highoil-content cultivars.

关 键 词：Brassica napus Oil content QTL mapping RESEQUENCING TRANSCRIPTOME 

分 类 号：S565.4[农业科学—作物学]