Stable major QTL on chromosomes A07 and A08 increase shelling percentage in peanut(Arachis hypogaea L.)  

作　　者：Weitao Li Nian Liu Li Huang Yuning Chen Jianbin Guo Bolun Yu Huaiyong Luo Xiaojing Zhou Dongxin Huai Weigang Chen Liying Yan Xin Wang Yong Lei Boshou Liao Huifang Jiang 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062,Hubei,China

出　　处：《The Crop Journal》2022年第3期820-829,共10页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(31870319,31871666,and 31801403);China Agriculture Research System(CARS-13);National Program for Crop Germplasm Protection of China(2020NWB033);National Crop Germplasm Resources Center(NCGRC-2020-036);Central Public-interest Scientific Institution Basal Research Fund(Y2021CG05)。

摘　　要：Peanut is a major oilseed and food legume.Shelling percentage(SP),closely associated with seed yield,is a trait whose improvement is a major goal of peanut breeding.In this study,a mapping population(Xuhua 13×Zhonghua 6)was used to map quantitative trait loci(QTL)controlling SP in four environments.Two stable major QTL for SP were mapped on both SSR-and SNP-based genetic maps.q SPA07.1 on chromosome A07 explained up to 31.7%of phenotypic variation,and q SPA08.2 on chromosome A08 explained up to 10.8%.Favorable alleles of q SPA07.1 and q SPA08.2 were derived from the female and male parents,respectively.Eight recombinant inbred lines(RILs)carrying both favorable alleles showed superiority in SP over the two parents in all environmental trials.A combination of the two favorable alleles using the linked markers was verified to increase SP by~5%in the RIL population and by~3%SP in diverse peanut cultivars.q SPA07.1 and q SPA08.2 were delimited to respectively a 0.73-Mb interval harboring 96 genes and a 3.93-Mb interval harboring 238 genes.Respectively five and eight genes with high expression in pods,including enzymes and transcription factors,were assigned as candidate genes for q SPA07.1 and q SPA08.2.These consistent major QTL provide an opportunity for fine mapping of genes controlling SP,and the linked markers may be useful for genetic improvement of SP in peanut.

关 键 词：PEANUT Shelling percentage QTL mapping Genomic region Candidate genes InDel marker 

分 类 号：S565.2[农业科学—作物学]