Neuroprotective effects of exogenous brain-derived neurotrophic factor on amyloid-beta 1-40-induced retinal degeneration  

作　　者：Mohd Aizuddin Mohd Lazaldin Igor Iezhitsa Renu Agarwal Puneet Agarwal Nafeeza Mohd Ismail 

机构地区：[1]Department of Biosciences,Faculty of Science,Universiti Teknologi Malaysia,Johor Bahru,Malaysia [2]School of Medicine,International Medical University,Kuala Lumpur,Malaysia [3]Department of Pharmacology and Bioinformatics,Volgograd State Medical University,Volgograd,Russia [4]Faculty of Medicine,Universiti Teknologi MARA,Sungai Buloh Campus,Selangor,Malaysia

出　　处：《Neural Regeneration Research》2023年第2期382-388,共7页中国神经再生研究（英文版）

基　　金：supported by the Ministry of Higher Education,Government of Malaysia,No.FRGS/2/2014/SG03/UITM/02/2 UiTM IRMI file No.600-RMI/FRGS 5/3(111/2014),toⅡ;Yayasan Penyelidikan Otak,Minda dan Neurosains Malaysia(YPOMNM),No.YPOMNM/2019-04(2)UiTM IRMI No.100-IRMI/PRI 16/6/2(010/2019),to MAML。

摘　　要：Amyloid-beta(Aβ)-related alterations,similar to those found in the brains of patients with Alzheimer's disease,have been observed in the retina of patients with glaucoma.Decreased levels of brain-derived neurotrophic factor(BDNF)are believed to be associated with the neurotoxic effects of Aβpeptide.To investigate the mechanism underlying the neuroprotective effects of BDNF on Aβ_(1-40)-induced retinal injury in Sprague-Dawley rats,we treated rats by intravitreal administration of phosphate-buffered saline(control),Aβ_(1-40)(5 nM),or Aβ_(1-40)(5 nM)combined with BDNF(1μg/mL).We found that intravitreal administration of Aβ_(1-40)induced retinal ganglion cell apoptosis.Fluoro-Gold staining showed a significantly lower number of retinal ganglion cells in the Aβ_(1-40)group than in the control and BDNF groups.In the Aβ_(1-40)group,low number of RGCs was associated with increased caspase-3 expression and reduced TrkB and ERK1/2 expression.BDNF abolished Aβ_(1-40)-induced increase in the expression of caspase-3 at the gene and protein levels in the retina and upregulated TrkB and ERK1/2 expression.These findings suggest that treatment with BDNF prevents RGC apoptosis induced by Aβ_(1-40)by activating the BDNF-TrkB signaling pathway in rats.

关 键 词：amyloid-beta 1-40 brain-derived neurotrophic factor FLUORO-GOLD neuroprotection retinal ganglion cells(RGC) retinal toxicity tropomyosin receptor kinase B(TrkB) 

分 类 号：R774.13[医药卫生—眼科]