β-Estradiol 17-acetate enhances the in vitro vitality of endothelial cells isolated from the brain of patients subjected to neurosurgery  

作　　者：Sonia Guzzo Pasquale De Bonis Barbara Pavan Luciano Fadiga 

机构地区：[1]Department of Neuroscience and Rehabilitation,Section of Physiology,University of Ferrara,Ferrara,Italy [2]Center for Translational Neurophysiology of Speech and Communication(CTNSC),Italian Institute of Technology(IIT),Ferrara,Italy [3]Department of Translational Medicine and for Romagna,University of Ferrara,Ferrara,Italy

出　　处：《Neural Regeneration Research》2023年第2期389-395,共7页中国神经再生研究（英文版）

基　　金：supported by EnTimeMent H2020-FETPROACT-824160(to LF)。

摘　　要：In the current landscape of endothelial cell isolation for building in vitro models of the blood-brain barrier,our work moves towards reproducing the features of the neurovascular unit to achieve glial compliance through an innovative biomimetic coating technology for brain chronic implants.We hypothesized that the autologous origin of human brain mic rovascular endothelial cells(hBMECs)is the first requirement for the suitable coating to prevent the glial inflammato ry response trigge red by foreign neuroprosthetics.Therefo re,this study established a new procedure to preserve the in vitro viability of hBMECs isolated from gray and white matter specimens taken from neurosurge ry patients.Culturing adult hBMECs is generally considered a challenging task due to the difficult survival ex vivo and progressive reduction in proliferation of these cells.The addition of 10 nMβ-estradiol 17-acetate to the hBMEC culture medium was found to be an essential and discriminating factor promoting adhesion and proliferation both after isolation and thawing,suppo rting the well-known protective role played by estrogens on microvessels.In particular,β-estradiol 17-acetate was critical for both freshly isolated and thawed female-derived hBMECs,while it was not necessary for freshly isolated male-derived hBMECs;however,it did countera ct the decay in the viability of the latter after thawing.The tumo r-free hBMECs were thus cultured for up to 2 months and their growth efficiency was assessed befo re and after two periods of cryopreservation.Des pite the thermal stress,the hBMECs remained viable and suitable for re-freezing and storage for several months.This approach increasing in vitro viability of hBMECs opens new perspectives for the use of cryopreserved autologous hBMECs as biomimetic therapeutic tools,offering the potential to avoid additional surgical sampling for each patient.

关 键 词：β-estradiol 17-acetate 17Β-ESTRADIOL CRYOPRESERVATION GENDER-SPECIFIC gray matter human brain microvascular endothelial cells surgical resections vascular protection white matter 

分 类 号：R741[医药卫生—神经病学与精神病学]