高效液相色谱法(HPLC)测定芒果多种植物生长调节剂含量  

Determination of contents of several plant growth regulators in mango by high performance liquid chromatography(HPLC)

作　　者：王丽荣 贾文君[1,3] 陈明敏 邵远志 李雯[1,3] WANG Lirong;JIA Wenjun;CHEN Mingmin;SHAO Yuanzhi;LI Wen(College of Horticulture,Hainan University,Haikou 570228,China;College of Life Sciences,Hainan University,Haikou 570228,China;Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Haikou 570228,China)

机构地区：[1]海南大学园艺学院,海口570228 [2]海南大学生命科学学院,海口570228 [3]海南省热带园艺作物品质调控重点实验室,海口570228

出　　处：《植物生理学报》2022年第5期981-988,共8页Plant Physiology Journal

基　　金：国家自然科学基金(32072275)。

摘　　要：为了能高效、快速检测果实中的多种植物生长调节剂含量,本文以‘贵妃’芒果为材料,以6-苄氨基嘌呤(6-BA)、噻苯隆(TDZ)、氯吡脲(CPPU)、赤霉素(GA3)和多效唑(PBZ)5种植物生长调节剂为检测对象,通过对色谱条件的优化,建立了利用高效液相色谱仪(HPLC)结合紫外检测器的分析方法。该方法能够高效、准确地检测不同产地、不同采收成熟度和采后处理芒果果实中的多种植物生长调节剂含量,研究结果不仅可为生产中准确检测植物生长调节剂提供科学方法,也为合理施用植物生长调节剂提供理论指导。In order to efficiently and quickly detect the content of multiple plant growth regulators in the fruit at the same time,this article uses the’Guifei’mango as the material,5 plant growth regulators including 6-benzylaminopurine(6-BA),thidiazuron(TDZ),and forchlorfenuron(CPPU),gibberellin(GA3)and paclobutrazol(PBZ)were detected.An analytical method using high performance liquid chromatography(HPLC)combined with UV detector was established after optimizing chromatographic conditions.This method can efficiently and accurately detect the content of 5 plant growth regulators in mango fruits of different origins,different harvest maturities and post-harvest treatments.The results can not only provide scientific methods for the accurate detection of plant growth regulators in production,but also provide theoretical guidance for the rational application.

关 键 词：高效液相色谱法 植物生长调节剂 芒果果实 

分 类 号：S667.7[农业科学—果树学]