Nanocarrier-delivered dsRNA suppresses wing development of green peach aphids  

作　　者：Yun-Hui Zhang Zhong-Zheng Ma Hang Zhou Zi-Jian Chao Shuo Yan Jie Shen 

机构地区：[1]Department of Plant Biosecurity and MOA Key Lab of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University,Beijing,China

出　　处：《Insect Science》2022年第3期669-682,共14页昆虫科学（英文版）

基　　金：We would like to acknowledge the National Natural Science Foundation of China(32030012 and 31900363);Beijing Natural Science Foundation(6204043);National Key Research and Development Program of China(2018YFD0200804).

摘　　要：RNA interference(RNAi)has developed rapidly as a potential“green”pest management strategy.At present,most studies have focused on the screening of aphid lethal genes,whereas only a few studies have been conducted on wing development,which is crucial for aphid migration and plant-virus dissemination.Here,the Myzus persicae genes vestigial(vg)and Ultrabithorax(Ubx)related to wing development,were cloned.These two genes were expressed in various tissues of 3rd-instar winged aphids.The mRNA level of vg was high in 3rd-instar nymphs,whereas the expression level of Ubx was high in adults.The nanocarrier-mediated delivery system delivered double-stranded RNAs for aphid RNAi using topical and root applications.The expression levels of vg and Ubx were downregulated by 44.0%and 36.5%,respectively,using the topical application.The simultaneous RNAi of the two target genes caused 63.3%and 32.2%wing aberration rates using topical and root applications,respectively.The current study provided a promising method for controlling aphid migration to alleviate the spread of insect transmitted plant diseases.

关 键 词：NANOCARRIER RNA interference ULTRABITHORAX VESTIGIAL wing development 

分 类 号：Q96[生物学—昆虫学]