Genome-wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura  

作　　者：Qilin Li Mengge Li Mengyao Zhu Jielai Zhong Liang Wen Jie Zhang Ruonan Zhang Qiang Gao Xiao-Qiang Yu Yuzhen Lu 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,510631,China [2]College of Biology,Hunan University,Changsha,410082,China

出　　处：《Insect Science》2022年第3期783-800,共18页昆虫科学（英文版）

基　　金：This research is supported by the National Key R&D Program of China(No.2019YFD1002100);National Natural Science Foundation of China(No.31901941 and No.31970474);Hunan Provincial Natural Science Foundation of China(2020JJ4178).

摘　　要：Cadherin,aminopeptidase N(APN)and alkaline phosphatase(ALP)have been characterized as Cry receptors.In this study,comparative genomic analysis of the 3 receptor families was performed in 7 insects.ALPs and APNs are divided into three and eight clades in phylogenetic trees,respectively.ALPs in clade 3 and APNs in clade 1 contain multiple paralogs within each species and most paralogs are located closely in chromosomes.Drosophila melanogaster has expanded APNs in clade 5 and were lowly expressed in midgut.Cadherins are divided into 16 clades;they may diverge before holometabolous insect speciation except for BtR and Cad89D-like clades.Eight insects from different orders containing BtR orthologs are sensitive to Cryl A or Cry3A,while five species without BtR are insensitive to both toxins.Most APNs in clade 1,several ALPs in clade 3,BtR and Cad89D-like genes were highly or moderately expressed in larval midgut of Spodoptera litura and the other six species,and several members in these clades have been identi-•fied as Cry receptors.Expressions of putative 5.litura Cry receptors in the midgut after exposing to Bt toxins were also analyzed.

关 键 词：alkaline phosphatase aminopeptidase N Bacillus thuringiensis CADHERIN Cry toxin 

分 类 号：Q96[生物学—昆虫学]