干露胁迫对毛蚶稚贝生理代谢相关酶活力的影响  

Effects of air exposure on enzyme activity related to physiological metabolism in juvenile Scapharca kagoshimensis

作　　者：张扬 江天棋 姜亚洲[2] 凌建忠[2] 孙鹏[2] 唐保军[2] ZHANG Yang;JIANG Tianqi;JIANG Yazhou;LING Jianzhong;SUN Peng;TANG Baojun(College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306 , China;2 . East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院东海水产研究所,上海200090

出　　处：《海洋渔业》2022年第2期219-227,共9页Marine Fisheries

基　　金：国家重点研发计划项目(2020YFD0900804);上海市自然科学基金(18ZR1450000);国家贝类产业体系建设项目(CARS-49)。

摘　　要：为研究干露对毛蚶(Scapharca kagoshimensis)稚贝生理代谢的影响,利用差减法测定了干露2、4、8、12、24、36、48、72 h后毛蚶稚贝的失水率,以及干露0、2、6、12、24、48 h后全组织丙酮酸激酶(PK)、己糖激酶(HK)、琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)活力和三磷酸腺苷(ATP)含量。结果显示,持续干露48 h后稚贝失水率达32.4%,死亡率超过33.3%;PK、SDH、ACP活力总体呈现先升高后下降的趋势,分别在24、12、6 h达到最大值;SOD和HK的活力分别在6~24 h、6~12 h显著低于对照组;组织ATP含量在干露后迅速升高,12 h后降至对照组水平,表明稚贝可能由有氧代谢转为无氧代谢。为评估毛蚶稚贝干露胁迫后耗氧率和滤水率的恢复情况,将干露48 h的毛蚶稚贝再放入20℃水中,测定放回后6、12、24、48 h的滤水率和耗氧率。结果显示,滤水率在24 h前维持在较低水平,其后持续显著上升;耗氧率在12 h达到最小值后显著上升。研究表明,干露12~24 h是毛蚶稚贝代谢和相关酶活力变化较大的时期;毛蚶稚贝可调整摄食和生理代谢水平,使机体在干露胁迫后尽快恢复到正常状态。研究结果可为揭示毛蚶干露胁迫响应机制提供参考,对毛蚶的养殖和苗种运输也有指导作用。Air exposure is an important factor affecting the distribution,growth and survival of intertidal shellfish.Studying the physiological metabolism and related enzyme activity of intertidal shellfish under air exposure stress is helpful to understand the stress adaptation mechanism of marine shellfish.In order to explore the effect of air exposure on ark shell Scapharca kagoshimensis,the study took ark shell juveniles in Xiangshan Bay as research object.The water loss rate of the juveniles was measured after 2,6,12,24,48,72 h of air exposure,and pyruvate kinase(PK),hexokinase(HK),succinate dehydrogenase(SDH),superoxide dismutase(SOD),acid phosphatase(ACP)activity,and adenosine triphosphate(ATP)content in whole tissues of the juveniles were also measured.Results showed that the water loss rate of juveniles reached 32.4%after 48 hours of continuous air exposure,and the mortality rate exceeded 33.3%.PK,SDH and ACP activity showed an overall trend of first increasing and then declining,reaching the maximum values at 24,12,6 h,respectively.The activity of SOD and HK was significantly lower than that of the control group during 6-24 h and 6-12 h,respectively.The ATP content in tissues increased rapidly after air exposure,and declined to the level of the control group after 12 h.Results indicated that:with the time of air exposure,the juveniles might regulate the metabolic mode and level by regulating the activity of metabolic enzymes to increase the anaerobic oxidation of glucose to produce ATP,providing energy for the body to resist the air exposure stress.Under air exposure stress,the respiratory metabolism of juveniles was closely related to their immune functions.In order to evaluate the recovery of oxygen consumption rate and filtration rate after air exposure stress,the juveniles after 48 h air exposure were then put back into seawater at 20℃.The filtration rate and oxygen consumption rate were measured at 6,12,24,48 h after being put back.Results showed that the filtration rate was maintained at a low level in th

关 键 词：干露 毛蚶 代谢 酶活力 滤水率 耗氧率 

分 类 号：S917.4[农业科学—水产科学]