水温升高对黑斑侧褶蛙蝌蚪变态时长和血液参数的影响  

Effects of increased water temperature on the metamorphosis duration and hematological parameters in tadpoles of Pelophylax nigromaculatus

作　　者：张志强[1] 杨雨晴 陈利 金冰艳 王佳慧 刁迎珠 伍亮 ZHANG Zhiqiang;YANG Yuqing;CHEN Li;JIN Bingyan;WANG Jiahui;DIAO Yingzhu;WU Liang(School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2022年第2期254-258,共5页Journal of Anhui Agricultural University

基　　金：安徽农业大学2020年国家级(202010364072);2019年省级(201910364036)大学生创新创业训练计划项目创新训练项目共同资助。

摘　　要：为探究升温对黑斑侧褶蛙(Pelophylax nigromaculatus)变态时长、形态表型和血液参数的影响,在水温为21、23、25和27℃条件下,测定了从37期蝌蚪发育至46期变态完成期时的变态时长、身体大小、血液中各型白细胞的百分比及嗜中性粒细胞与淋巴细胞的比值(N/L的比值)的变化。结果显示,21℃组和23℃组的变态时长显著长于25℃组和27℃组(P<0.05);体重、体长以及体重与体长的比值都在21℃组最高,25℃组最低,但均无明显的组间差异(P>0.05)。水温对血液淋巴细胞、嗜中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和单核细胞的百分比以及N/L的比值均无明显影响(P>0.05)。升温可加速黑斑侧褶蛙蝌蚪的变态过程,但不影响各型白细胞所代表的天然免疫能力和应激反应能力。To explore the effects of water temperature on metamorphosis duration,morphological phenotypes and hematological indices,under the conditions of the different water temperatures(21,23,25 and 27℃),tadpoles at developmental duration from Gosner 37-stage to 46-stage,body size and different subtypes of leukocyte in the circulation blood,as well as the ratio of neutrophils to lymphocytes(ratio of N/L)were measured while the tadpoles of Pelophylax nigromaculatus reached the metamorphosis completion period.The results were as follows:Metamorphosis duration in the 21℃and 23℃groups was significantly longer than that of 25℃and 27℃groups(P<0.05);body mass,snout to vent length,and the ratio of body mass to snout to vent length showed the highest value in the 21℃group,lowest in the 25℃group,but no significant group differences were found(P>0.05).Different water temperature treatments had no great impact on percentages of lymphocytes,neutrophils,eosinophils,basophils and monocytes,as well as the ratio of N/L(P>0.05).Increased water temperature could accelerate the metamorphosis process,but it had no obvious effect on innate immunity represented by different subtypes of leukocyte and the ability of stress response.

关 键 词：黑斑侧褶蛙 变态时长 蝌蚪 温度 各型白细胞的百分比 应激 

分 类 号：S966.3[农业科学—水产养殖] Q959.5[农业科学—水产科学]