Rhodotorula mucilaginosa PA-1合成硒化银量子点及其抗菌活性  

The biosynthesis of silver selenide quantum dots by Rhodotorula mucilaginosa PA-1 and its antibacterial activity

作　　者：任倩文 鲍巧雨 鲍中康 张友德 武超[1,2] 邓国志 REN Qianwen;BAO Qiaoyu;BAO Zhongkang;ZHANG Youde;WU Chao;DENG Guozhi(School of Resources and Environmental Engineering,Anhui University,Hefei 230601;Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration(Anhui University),Hefei 230601;Anhui Xin Yu Ecological Industry Co.,Ltd.,Hefei 230601)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601 [2]湿地生态保护与修复安徽省重点实验室(安徽大学),合肥230601 [3]安徽新宇生态产业股份有限公司,合肥230601

出　　处：《安徽农业大学学报》2022年第2期293-298,共6页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(31670126);湿地生态保护与修复安徽省重点实验室开放基金(AKLWEPR-K-2020-02);安徽省住房城乡建设科学技术计划项目(2017YF-03)共同资助。

摘　　要：硒化银量子点是一种半导体材料,具有特殊的光学和电子特性,因此在许多领域得到了越来越广泛的应用。与化学合成相比,生物合成硒化银量子点具有许多优点,尺寸均匀,绿色安全。以Rhodotorula mucilaginosa PA-1为原料,在好氧条件下生物合成硒化银量子点,并通过光致发光光谱(PL)和荧光显微分析对其进行了表征;透射电子显微镜(TEM)分析硒化银量子点的尺寸和形貌。XPS和XRD分析硒化银量子点的元素价态和晶型。目前,微生物感染已对公众健康构成严重威胁,而卫生机构及其周围环境滥用抗生素导致的多重耐药现象更是加剧了这一威胁。金属纳米颗粒可以诱导细胞死亡,但毒性效应通常是非特异性的。结果以Escherichia coli ATCC 25922为模型致病菌时,发现硒化银量子点可以杀死大量的细菌细胞,具有较好的抗菌活性。Silver selenide quantum dots are a kind of semiconductor material with special optical and electronic properties, so they have been used more and more widely in many fields. Compared with chemical synthesis, biosynthesis of silver selenide quantum dots has many advantages, such as uniform size, green and safe. In this study, silver selenide quantum dots(QDs) were biosynthesized from colloidal Rhodotorula mucilaginosa PA-1 under aerobic conditions and characterized by photoluminescence(PL)and microscopic analysis;the size and morphology of silver selenide quantum dots were evaluated by transmission electron microscopy(TEM), and the valence states and crystal forms of silver selenide quantum dots were evaluated by X-ray electron spectroscopy(XPS) and X-ray electron diffraction(XRD). At present, microbial infection has posed a serious threat to public health, which is exacerbated by the phenomenon of multiple drug resistance caused by the abuse of antibiotics in health institutions and their surrounding environment. Metal nanoparticles can induce cell death, but the toxic effects are usually nonspecific. Here, we took Escherichia coli ATCC25922 as a model pathogen and found that silver selenide quantum dots can kill most of bacterial cells with good antibacterial activity.

关 键 词：生物合成 硒化银 量子点 Rhodotorula mucilaginosa PA-1 抗菌活性 

分 类 号：X172[环境科学与工程—环境科学]