基于碳点的比率荧光探针用于检测6-硫鸟嘌呤  

A ratiometric fluorescence probe based on carbon dots for the detection of 6-thioguanine

作　　者：梁志钢 任丹丹 程霞 岑瑶 许贯虹[1] 魏芳弟[1] 胡琴[1] LIANG Zhigang;REN Dandan;CHENG Xia;CEN Yao;XU Guanhong;WEI Fangdi;HU Qin(School of Pharmacy,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学药学院,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2022年第6期830-836,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81973283,61775099,21705080)。

摘　　要：目的:基于临床常用抗肿瘤药物6-硫鸟嘌呤(6-thioguanine,6-TG)中硫原子与Cu^(2+)的高度亲和力,构建了一种新的比率荧光方法,用于检测抗肿瘤药物6-TG。方法:邻苯二胺(o-phenylenediamine,OPD)能够被游离Cu^(2+)氧化成560 nm处发黄色荧光的产物2,3-二氨基酚嗪(2,3-diaminophenazine,DAP),体系中450 nm处发射蓝色荧光的碳点与DAP之间存在荧光内滤效应,因此DAP能够使碳点荧光猝灭。当目标物6-TG存在时,会与OPD竞争Cu^(2+),导致氧化产物DAP减少,使其黄色荧光降低,和碳点之间的内滤效应减弱,碳点的蓝色荧光恢复,荧光强度比值F_(450)/F_(560)与6-TG的浓度呈线性关系,因此该探针可用于定量检测6-TG。结果:随着6-TG浓度的增加,该体系荧光强度比值F_(450)/F_(560)逐渐增强,在最佳反应条件下,6-TG的线性范围为40.00~160.00μmol/L,检测限为61.10 nmol/L,相关系数R^(2)=0.992。结论:该方法选择性好、准确度高、操作简便、经济实用,可应用于6-TG药片含量测定。Objective:Based on the high affinity between Cu^(2+)and sulfur atom in clinical commonly used anti-cancer drug 6-thioguanine(6-TG),a novel ratiometric fluorescence method was constructed for the detection of 6-TG.Methods:The o-phenylenediamine(OPD)could be oxidized by Cu^(2+)to its yellow fluorescence product 2,3-diaminophenazine(DAP)at 560 nm.The fluorescence intensity of carbon dot(CD)at 450 nm could be quenched by DAP because of the inner filter effect(IFE).However,in the presence of 6-TG,it would compete with OPD for binding Cu^(2+),resulting in the reduction of the oxidation product DAP and its yellow fluorescence.Therefore,the IFE between the CD and DAP was weakened,and the blue fluorescence of the CD was restored.The fluorescence intensity ratio(F_(450)/F_(560))was linearly correlated with the concentration of 6-TG.Therefore,this probe could be used to quantitatively detect 6-TG.Results:With the increase of 6-TG,the fluorescence intensity ratio(F_(450)/F_(560))was gradually enhanced.Under the optimal reaction conditions,the linear concentration of 6-TG ranged from 40.00 to 160.00μmol/L with detection limits of61.10 nmol/L,and the correlation coefficient R^(2) was 0.992.Conclusion:This probe possesses good selectivity,high accuracy and is easy to operate.In addition,it is practical and economical,and can be applied to determine the content of 6-TG tablet.

关 键 词：6-硫鸟嘌呤 邻苯二胺 2 3-二氨基酚嗪 碳点 比率荧光探针 

分 类 号：R927.2[医药卫生—药学]