慢病毒沉默PRDX4抑制肺癌细胞A549迁移和侵袭及其机制的探讨  

Inhibition of migration and invasion of lung cancer cell line A549 by lentivirus silencing PRDX4 and it machanisms

作　　者：郭健强 周家伟 王清森 胡春晓 刘亚峰 苏忆欣 张文婷 王雪芹 吴静[1,3,4] 胡东 GUO Jianqiang;ZHOU Jiawei;WANG Qingsen;HU Chunxiao;LIU Yafeng;SU Yixin;ZHANG Wenting;WANG Xueqin;WU Jing;HU Dong(School of Medicine,Anhui University of Technology,Anhui Huainan 232000,China;Cancer Hospital Affiliated to Anhui University of Technology,Huainan Oriental Hospital Group Cancer Hospital,Anhui Huainan 232000,China;Anhui Occupational Health and Safety Engineering Laboratory,Anhui Huainan 232000,China;Anhui Key Laboratory of Industrial Dust Deep Purification and Occupational Health,Ministry of Education,Anhui Huainan 232000,China)

机构地区：[1]安徽理工大学医学院,安徽淮南232000 [2]安徽理工大学附属肿瘤医院/淮南东方医院集团肿瘤医院,安徽淮南232000 [3]安徽省职业健康安全工程实验室,安徽淮南232000 [4]安徽工业粉尘深度净化与职业健康教育部重点实验室,安徽淮南232000

出　　处：《现代肿瘤医学》2022年第13期2299-2304,共6页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81971483);安徽省高校协同创新项目(编号:GXXT-2020-058);安徽理工大学研究生创新创业项目(编号:2021CX2125,2021CX2126,2021CX2124,2020CX2083,2020CX2084)。

摘　　要：目的:研究慢病毒沉默过氧化物还原酶4(peroxiredoxin 4,PRDX4)对肺癌细胞迁移和侵袭能力的影响,并探讨其分子机制。方法:采用shRNA干扰技术敲低肺癌细胞系A549中PRDX4的表达,Western blot和qRT-PCR验证转染效果;划痕愈合实验检测细胞迁移能力;Transwell细胞侵袭实验检测细胞侵袭能力;Western blot分析p-ERK1/2、N-cadherin、Vimentin蛋白表达。结果:成功构建沉默PRDX4的肺癌细胞系;慢病毒感染后A549细胞PRDX4蛋白和mRNA表达水平显著低于对照组(P<0.05);与BC组和NC组相比,Sh组细胞迁移、侵袭能力显著降低(P<0.05);与BC组和NC组相比,Sh组细胞p-ERK1/2、N-cadherin、Vimentin蛋白表达水平显著降低。结论:沉默PRDX4可能通过ERK信号通路抑制肺癌细胞的迁移和侵袭。Objective:To study the effect of lentivirus silencing peroxiredoxin 4(PRDX4)on the migration and invasion of lung cancer cells and its molecular mechanism.Methods:The expression of PRDX4 in lung cancer cell line A549 was knocked down by shRNA interference,and the transfection effect was verified by Western blot and qRT-PCR.Cell migration was detected by scratch healing test.Transwell cell invasion test was used to detect the ability of cell invasion.The expressions of p-ERK1/2,N-cadherin and Vimentin were analyzed by Western blot.Results:A lung cancer cell line silencing PRDX4 was successfully constructed.After lentivirus infection,the expression levels of PRDX4 protein and mRNA in A549 cells were significantly lower than those in Sh group(P<0.05).Compared with BC group and NC group,the ability of cell migration and invasion in Sh group decreased significantly(P<0.05).Compared with BC group and NC group,the expression levels of p-ERK1/2,N-cadherin and Vimentin protein in Sh group were significantly lower.Conclusion:Silencing PRDX4 may inhibit the migration and invasion of lung cancer cells through ERK signaling pathway.

关 键 词：PRDX4 慢病毒载体 肺癌 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]