抗菌肽CC34的氨基酸从头替换设计及优化肽的生物活性检测  被引量:3

“De novo”Design for Peptide CC34 and Optimization of Bioactivity Detection of the Peptides

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作  者:张宇 姜宁[1] Zhang Yu;Jiang Ning(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区:[1]黑龙江八一农垦大学动物科技学院,大庆163319

出  处:《黑龙江八一农垦大学学报》2022年第3期67-75,共9页journal of heilongjiang bayi agricultural university

摘  要:以抗菌肽CC34的氨基酸序列为模板,采用氨基酸从头替换的方式对序列进行设计,借助生物信息学方法对预测不同序列的抗菌肽生物学参数,选取优化肽CC34PA、CC34PL、CC34K11、CC34P11进行化学合成,鉴定抗菌肽的抑菌活性、检测最小抑菌浓度、杀菌浓度和溶血率。结果表明,四种肽均属于α螺旋型抗菌肽,具有比CC34更好的非完美两亲性,对革兰氏阳性菌和阴性菌有抑制作用,除对产气肠杆菌的作用效果不及CC34之外,优化肽对不同的试验菌种的抑菌作用均有有不同程度的提高。优化肽相比于原肽对绵羊红细胞的溶血率降低,溶血率最低的是CC34K11;对鸡红细胞溶血率降低,溶血率最低的是CC34PA;对人红细胞溶血率没有降低作用。In this study,the amino acid sequence of CC34 was used as a template to design the sequence by"de novo"design.With the help of bioinformatics methods,the biological parameters of antibacterial peptides of different sequences were predicted,and the optimized peptides CC34PA,CC34PL,CC34K11 and CC34P11 were selected for chemical synthesis.The antimicrobial activity,minimum inhibitory concentration,bactericidal concentration and hemolysis rate of the antimicrobial peptides were determined.The results showed that the four peptides were all α helical antibacterial peptides,which had better imperfect amphiphilic activity than CC34,and had inhibitory effects on gram-positive and gram-negative bacteria.Except for ENTERobacter aerogenes,the inhibitory effects of the optimized peptides on different strains were improved to varying degrees.The hemolysis rate of the optimized peptide was lower than that of the original peptide,and CC34K11 had the lowest hemolysis rate.The hemolysis rate of chicken red blood cells decreased,and the lowest hemolysis rate was CC34PA and did not reduce the hemolysis rate of human red blood cells.

关 键 词:抗菌肽 从头替换 抑菌活性 抑菌浓度 杀菌浓度 溶血率 

分 类 号:S816.73[农业科学—饲料科学]

 

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