基于线粒体基因组的鲹科鱼类进化分析  

Evolution Analysis of Members in Family Carassiidae Based on Mitochondrial Genome

作　　者：王海山 蒋欢 陈治 杨超杰 叶乐 WANG Haishan;JIANG Huan;CHEN Zhi;YANG Chaojie;YE Le(College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya 572022,China;School of Marine Science,Ningbo University,Ningbo 315211,China)

机构地区：[1]海南热带海洋学院水产与生命学院,海南三亚572022 [2]宁波大学海洋学院,浙江宁波315211

出　　处：《水产学杂志》2022年第2期14-20,共7页Chinese Journal of Fisheries

基　　金：海南热带海洋学院博士科研启动基金项目(RHDXB201621).

摘　　要：用生物信息学方法研究了鲈形目鲹科(Carangidae)鲹亚科、鲳鲹亚科和鰤亚科中33种鱼的线粒体基因组,分别使用ClustalⅩ、MEGAⅩ和DnaSP 5.0软件进行核酸序列比对,碱基组成及核酸多样性分析和构建系统进化树。结果表明:1)33种鲹科鱼类线粒体基因组序列全长为16530~16609 bp,总的简约信息位点共6297个,变异位点共7072个(占42.0%);核苷酸组成A+T的含量占53.8%,大于G+C含量,呈AT偏向性;转换/颠换比值(R)1613/1026;2)33种鲹科鱼类种间遗传距离平均值为0.183;种间遗传距离最大值为卵形鲳鲹(Trachinotus ovatus)与高体若鲹(Carangoides equula)间的0.250,最小值为黑纹小条鰤(Seriolina nigrofasciata)与白舌尾甲鲹(Uraspis helvola)间的0.002;3)卵形鲳鲹与鲳鲹属外物种的遗传距离最大,平均值为0.229,泰勃圆鲹(Decapterus tabl)与其他鲹科鱼类平均遗传距离最小为0.170;4)若鲹属的高体若鲹与圆鲹属、竹筴鱼属聚为一支,与同属甲若鲹(Carangoides armatus)和马拉巴若鲹(Carangoides malabaricus)亲缘关系较远。鲳鲹属在进化上与其他鲹科鱼类分化较大,圆鲹属与其他鲹科鱼类遗传距离更近,在线粒体基因组水平上高体若鲹与圆鲹属鱼类亲缘关系更近,与传统分类结果存在差异。In this study,mitochondrial genomes were analyzed in 33 species in Caranginae,Chorineminae,and Seriolinae in Carangidae,one of the economically important groups in Perciformes fish,with bioinformatics method.DNA sequence alignment,mitochondrial genome nucleotide composition,variation loci and phylogenetic trees were reconstructed using ClustalⅩ,MEGAⅩand DnaSP 5.0 software.The results showed that the total length of the mitochondrial genome sequences of Carangidae fish was ranged from 16,530 to 16,609 bp,and 6,297 parsimony informative sites and 7,072 variation point were detected,variation point accounting for 42.0%.The content of nucleotides A+T accounted for 53.8%,greater than that of G+C,showing AT bias,with convert/transversion ratio(R)of 1613/1026.There was mean genetic distance of 0.183 in 33 species in Carangidae,with the maximal genetic distance of 0.250 in ovate pompano Trachinotus ovatus and jack Carangoides equula,and the minimum genetic distance of 0.002 in black-banded amberjack Seriolina nigrofasciata and cottonmouth jack Uraspis helvola.Ovate pompano had the maximal average genetic distance of 0.229 with other species,redtai scad Decapterus tabl had the minimum average genetic distance of 0.170 with other species.Jack Carangoides equula was shown to be a mixed branch with Decapterus and Trachurus,far from armed trevally C.armatus and cavalla C.malabaricus.

关 键 词：线粒体DNA 鲹科鱼类 系统进化 

分 类 号：S917[农业科学—水产科学]