新型过氧化氢生物发光探针的合成与光学性质评价  

Synthesis and optical properties of a novel bioluminescent probe for hydrogen peroxide detection

作　　者：盖文睿 胡世龙 康婷 海澳 柯博文[1] GAI Wenrui;HU Shilong;KANG Ting;HAI Ao;KE Bowen(Laboratory of Anaesthesiology and Critical Care Medicine,Translational Neuroscience Center,West China Hospital,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西医院转化神经科学中心麻醉与危重急救研究室,四川成都610041

出　　处：《华西药学杂志》2022年第3期225-228,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81773685);西藏自治区科技计划项目(编号:XZ201901-GB-08);成都市科技项目(编号:2018-CY02-00042-GX)。

摘　　要：目的设计并合成一个用于过氧化氢(H_(2)O_(2))可视化检测的新型小分子生物发光探针(HPBP)。方法在萤火虫萤光素的6’位羟基引入特异性识别基团芳基硼酸酯,构建H_(2)O_(2)小分子探针HPBP。通过探针与底物反应后光学信号强度的变化,对H_(2)O_(2)检测的特异性和灵敏度进行考察。结果成功制备了HPBP,该探针对低浓度H_(2)O_(2)(0.25~5μmol·L^(-1))的响应具有良好的浓度依赖性,且在H_(2)O_(2)浓度低于其他物质20~300倍时,发光强度仍为其他物质的2~5倍。结论制备的HPBP探针检测限低,特异性强,为研究H_(2)O_(2)在疾病发生发展过程中的机制和作用提供了有力工具。OBJECTIVE To develop a novel bioluminescent probe(HPBP)for visual detection of hydrogen peroxide(H_(2)O_(2)).METHODS The probe HPBP was synthesized by introducing an aryl boronate group to the 6’-hydroxy group of D-luciferin.The specificity and sensitivity of probe HPBP were evaluated by monitoring the fluctuation of luminescent intensity after triggered by H_(2)O_(2).RESULTS The bioluminescent probe HPBP was designed for the chemoselective visualization of low concentration H_(2)O_(2)(0.25-5μmol·L^(-1)).There is a good linearity between the bioluminescence intensities and H_(2)O_(2)levels.Moreover,the luminescent intensity generated from the reaction of the probe with H_(2)O_(2)was 2-5 times higher than that with other substances,where H_(2)O_(2)concentration was 20-300 times lower.CONCLUSION The probe HPBP with low limit of detection and strong specificity is developed,providing a powerful tool for mechanism study of hydrogen peroxide in the occurrence and development of diseases.

关 键 词：过氧化氢 HPBP 生物发光 探针 萤光素-萤光素酶系统 检测 萤火虫萤光素 2-溴甲基苯基硼酸频哪醇酯 

分 类 号：R914[医药卫生—药物化学]