川芎嗪通过调控mTOR的表达减轻细菌脂多糖诱导的脐静脉内皮细胞内皮屏障破坏  

Tetramethylpyrazine attenuates bacterial lipopolysaccharide-induced endothelial barrier disruption in umbilical vein endothelial cells by regulating mTOR expression

作　　者：高可飞 刘小碣 丁渡山 闵思敏 李言[3] 张永[1] GAO Ke-fei;LIU Xiao-jie;DING Du-shan;MIN Si-min;LI Yan;ZHANG Yong(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233000,China;Graduate School,Bengbu Medical College,Bengbu,Anhui 233000,China;Department of pathophysiology,Bengbu Medical College,Bengbu,Anhui 233000,China)

机构地区：[1]蚌埠医学院第一附属医院呼吸与危重症科,安徽蚌埠233000 [2]蚌埠医学院研究生院,安徽蚌埠233000 [3]蚌埠医学院病理生理教研室,安徽蚌埠233000

出　　处：《临床肺科杂志》2022年第7期1003-1009,共7页Journal of Clinical Pulmonary Medicine

基　　金：国家自然基金资助项目(No.81673791)。

摘　　要：目的观察川芎嗪(Tetramethylpyrazine,TMP)对细菌脂多糖(LPS)诱导的脐静脉内皮细胞屏障损伤(HUVEC)的保护作用,并探索可能的作用机制。方法将鉴定好的人脐带静脉内皮细胞根据加药处理因素不同,将实验分为空白对照组、LPS组,LPS+TMP组。对三组进行免疫荧光细胞骨架染色及细胞通透性实验、跨内皮电阻实验。应用Westernblot及qPCR方法进行mTOR表达量的定量分析。结果免疫荧光细胞骨架染色表明与空白组相比,LPS组应力纤维明显增多、增粗。而应用TMP治疗后应力纤维变少、变细。跨内皮电阻实验表明,加药刺激前三组电阻值差无统计学意义(P>0.05)。加入药物刺激后,LPS组较空白组,电阻值明显降低,而TMP组较LPS组明显增加(P<0.05)。细胞通透性实验表明加药刺激后,LPS组较空白组,通透系数明显增加,而TMP组较LPS组明显降低(P<0.05)。Westernblot及qPCR方法表明LPS组较空白组mTOR表达量增加,而TMP治疗之后mTOR表达量较LPS组下降。结论TMP可通过调控mTOR的表达量对LPS诱导的HUVEC内皮细胞屏障破坏起到保护作用。Objective To observe the protective effect of tetramethylpyrazine(TMP)on bacterial lipopolysaccharide(LPS)-induced umbilical vein endothelial cell barrier damage,and to explore the possible mechanism of action.Methods The identified human umbilical vein endothelial cells(HUVECs)were divided into blank control group,LPS group,and LPS+TMP group according to different drug treatment factors.Immunofluorescence cytoskeleton staining,cell permeability test,and transendothelial resistance test were performed in the three groups.The expression of mTOR was analyzed quantitatively by Western blot and qPCR.Results Immunofluorescence cytoskeleton staining showed that compared with the blank group,the stress fibers in the LPS group were significantly increased and thickened.However,after TMP treatment,the stress fibers became less and thinner.The transendothelial resistance test showed that there was no significant difference in the resistance values of the three groups before drug stimulation(P>0.05).After drug stimulation,the resistance value of the LPS group was significantly lower than that of the blank group.The TMP group was significantly higher than the LPS group(P<0.05).The permeability test showed that the permeability coefficient of the LPS group was significantly higher than that of the blank group,Compared with the LPS group,the TMP group was significantly lower(P<0.05).Western blot and qPCR showed that the expression of mTOR in the LPS group was higher than that in the blank group,while the expression of mTOR in the TMP group was lower than that in the LPS group.Conclusion TMP can protect HUVECs barrier damage induced by LPS by regulating the expression of mTOR.

关 键 词：川芎嗪 MTOR ALI HUVEC 细胞骨架 

分 类 号：R285[医药卫生—中药学]