以离子液体和甲酸铵为流动相添加剂的亲水作用色谱法分析生物胺  

Analysis of biogenic amines by hydrophilic interaction chromatography using ionic liquids and ammonium formate as mobile phase additives

作　　者：董淼鑫 万长长 殷振杰 马亚杰[1] 于泓[1] DONG Miao-xin;WAN Chang-chang;YIN Zhen-jie;MA Ya-jie;YU Hong(College of chemistry and Chemical Engineering,Harbin Normal University,Harbin 150025,China)

机构地区：[1]哈尔滨师范大学化学化工学院,黑龙江哈尔滨150025

出　　处：《化学研究与应用》2022年第6期1251-1258,共8页Chemical Research and Application

基　　金：黑龙江省教育厅高等学校基本科研业务费科研项目(2020KYYWF0361)资助。

摘　　要：发展了用吡啶离子液体和甲酸铵作为流动相添加剂的亲水作用色谱分离和紫外检测生物胺类物质的分析方法。研究了吡啶离子液体、咪唑离子液体和甲酸铵作为流动相添加剂对分析生物胺的影响,考察了吡啶离子液体的浓度、甲酸铵浓度、乙腈浓度以及检测波长等因素的影响,并讨论了离子液体的作用、保留规律和相关机理。用亲水作用色谱柱分离极性物质生物胺,吡啶离子液体和甲酸铵改善了生物胺的分离和检测。采用亲水作用色谱柱,以乙腈/0.5 mmol·L^(-1)N-乙基吡啶-1.0 mmol·L^(-1)甲酸铵混合水溶液(体积比25/75)为流动相,在柱温30℃,流速1.0 mL·min^(-1),紫外检测波长210 nm条件下,酪胺、苯乙胺和色胺在30 min内被完全分离检测,其检出限低于1.0 mg·L^(-1),线性和重现性良好。将此方法应用于面包发酵饮料样品中生物胺含量的测定,加标回收率为92.6%~102.0%。该方法简便、快捷,具有较好的实用性。Hydrophilic interaction chromatography with pyridinium ionic liquids and ammonium formate as mobile phase additives was developed for the separation and ultraviolet detection of biogenic amines.The influences of pyridinium ionic liquids,imidazolium ionic liquids and ammonium formate as mobile phase additives on the analysis of bioamines were studied.The effects of the concentrations of pyridinium ionic liquids,ammonium formate,acetonitrile,detection wavelength and other factors on the analysis of biogenic amines were investigated.The function of ionic liquids,retention rules and related mechanisms have been discussed.The hydrophilic interaction column is suitable for the separation of polar substance biogenic amines.The pyridinium ionic liquid and ammonium formate improved the separation and detection of biogenic amines.Tyramine,phenethylamine and tryptamine were completely separated and detected within 30 min using the hydrophilic interaction column and the mobile phase of acetonitrile/0.5 mmol·L^(-1)N-ethylpyridine-1.0 mmol·L^(-1)ammonium formate mixed solution(volume ratio 25/75)at the column temperature of 30°C,the flow rate of 1.0 mL·min^(-1)and the ultraviolet detection wavelength of 210 nm.The limits of detection were less than 1.0 mg·L^(-1).The linearity and reproducibility of the method are good.The method was applied to the determination of biogenic amine content in bread fermented beverage samples and the spiked recoveries ranged from 92.6%to 102.0%.This method is simple,rapid and has good practicability.The recoveries of standard addition were between92.6%and 102.0%.This method is simple and easy to apply.

关 键 词：离子液体 亲水作用色谱 生物胺 紫外检测 

分 类 号：O657.7[理学—分析化学]