电针神庭穴和百会穴对脑缺血/再灌注损伤后学习记忆障碍大鼠脑组织超氧化物歧化酶活性和丙二醛含量的影响研究  

Effect of Electroacupuncture at Shenting and Baihui Acupoints on Superoxide Dismutase Activity and Malondialdehyde Content in the Brain Tissue in a Rat Model of Learning and Memory Impairment Induced by Cerebral Ischemia-reperfusion Injury

作　　者：闫晓 LEE JAEMYUNG 张铭[2] 李瑞青[2] 高静 冯晓东 YAN Xiao;LEE JAEMYUNG;ZHANG Ming;LI Ruiqing;GAO Jing;FENG Xiaodong(School of Rehabilitation Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;Rehabilitation Center,the First Affiliated Hospital of Henan University of CM,Zhengzhou 450000,China)

机构地区：[1]河南中医药大学康复医学院,河南省郑州市450046 [2]河南中医药大学第一附属医院康复中心,河南省郑州市450000

出　　处：《中国全科医学》2022年第23期2892-2898,共7页Chinese General Practice

基　　金：国家自然科学基金资助项目(U2004131,82174473,82104973);河南省科技攻关计划项目(202102310167)。

摘　　要：背景自由基代谢异常是造成缺血性脑卒中患者认知障碍的病理机制之一,而电针神庭、百会穴可改善认知障碍患者的学习记忆能力。目的观察电针对脑缺血/再灌注损伤后学习记忆障碍大鼠脑组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量变化的影响,探讨电针改善脑缺血/再灌注损伤后学习、记忆功能障碍的可能机制。方法2019年8月至2020年8月,采用SPSS 22.0软件将18只雄性、SPF级SD大鼠进行完全随机化分组,分为假手术组(6只)、模型组(6只)和电针组(6只)。假手术组大鼠仅切开颈部皮肤,分离颈部动脉;造模大鼠采用大脑中动脉阻塞法制作脑缺血/再灌注损伤模型,在造模大鼠完全清醒后,按照Zea-Longa法筛选分值为1~3分的大鼠随机纳入模型组和电针组。于造模后1 d对电针组大鼠的神庭、百会穴施以疏密波,1 Hz/20 Hz、1次/d、30 min/次,共连续2周的电针治疗。模型组和假手术组大鼠只固定时间抓取,不给予任何干预措施。治疗结束后采用Morris水迷宫实验观察各组大鼠学习记忆能力的变化,采用总超氧化物歧化酶比色法测试盒(WST-1法)检测脑组织SOD活性,丙二醛比色法测试盒(TBA法)检测脑组织MDA含量。结果模型组和电针组大鼠均造模成功。模型组大鼠第13天逃避潜伏期长于假手术组,穿越平台次数少于假手术组(P<0.05);电针组大鼠第13天逃避潜伏期短于模型组,穿越平台次数多于模型组(P<0.05)。模型组大鼠SOD活性低于假手术组,MDA含量高于假手术组(P<0.05);电针组大鼠SOD活性高于模型组,MDA含量低于模型组(P<0.05)。结论电针神庭、百会穴可改善脑缺血/再灌注损伤后大鼠的学习记忆障碍,其机制可能与改善脑组织抗氧化酶SOD活性、减轻脂质过氧化产物MDA蓄积有关。Background The use of electroacupuncture at Shenting and Baihui acupoints may improve the learning and memory impairment in cerebral ischemic stroke patients with cognitive impairment induced by one pathogenic factor known as abnormal free radical metabolism.Objective To explore the possible mechanism of using electroacupuncture at Shenting and Baihui acupoints to improve the learning and memory impairment in a rat model of cerebral ischemia-reperfusion induced learning and memory impairment by observing the changes of superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in brain tissues of the model.Methods This experiment was conducted from August 2019 to August 2020.Eighteen SPF male SD rats were selected and equally randomized into three groups by use of random allocation using SPSS 22.0:sham operation group(receiving a neck incision for separating the neck artery,then being caught at a fixed time point),model group(receiving occlusion of the middle cerebral artery for inducing ischemia-reperfusion injury,then being assessed with a Zea-Longa score of 1-3 points in the fully awake state,and being caught at a fixed time point)and electroacupuncture group(first receiving interventions similar to those given to rats in the model group,then on the first day after being successfully modeled,receiving a 30-minute electroacupuncture at Shenting and Baihui acupoints with dense-sparse waves in the frequency range of 1 Hz/20 Hz,once daily,for two consecutive weeks).When the intervention ended,Morris water maze experiment was used to test the learning and memory ability of all rats.The SOD activity and MDA content in brain tissues of rats were measured by WST-1 assay,and thiobarbituric acid assay,respectively.Results The rats in both model group and electroacupuncture group were modeled successfully.On the 13th day of training trial,compared with rats in the sham operation group,those in the model group showed an increased escape latency and decreased number of crossing the platform(P<0.05);rats in the elect

关 键 词：脑缺血 再灌注损伤 电针 神庭 百会 超氧化物歧化酶 丙二醛 记忆障碍 模型 动物 

分 类 号：R743.31[医药卫生—神经病学与精神病学]