Indigenous microbiota protects development of medication-related osteonecrosis induced by periapical disease in mice  

作　　者：Wen Du Mengyu Yang Terresa Kim Sol Kim Drake W.Williams Maryam Esmaeili Christine Hong Ki-Hyuk Shin Mo K.Kang No-Hee Park Reuben H.Kim 

机构地区：[1]State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Prosthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu,China [2]The Shapiro Family Laboratory of Viral Oncology and Aging Research,Los Angeles,USA [3]Section of Restorative Dentistry,UCLA School of Dentistry,Los Angeles,USA [4]Department of Orofacial Sciences,UCSF School of Dentistry,San Francisco,USA [5]UCLA Jonsson Comprehensive Cancer Center,Los Angeles,USA [6]David Geffen School of Medicine at UCLA,Los Angeles,USA

出　　处：《International Journal of Oral Science》2022年第2期156-163,共8页国际口腔科学杂志（英文版）

基　　金：supported National Institutes of Health/National Institute of Dental and Craniofacial Research(grant DE023348 to R.H.K.,grant DE025172 to D.W.W.);China Postdoctoral Science Foundation(grant 2019M663526 to W.D.)。

摘　　要：Bacterial infection is a common finding in patients,who develop medication-related osteonecrosis of the jaw(MRONJ)by the longterm and/or high-dose use of anti-resorptive agents such as bisphosphonate(BPs).However,pathological role of bacteria in MRONJ development at the early stage remains controversial.Here,we demonstrated that commensal microbiota protects against MRONJ development in the pulp-exposed periapical periodontitis mouse model.C57/BL6 female mice were treated with intragastric broadspectrum antibiotics for 1 week.Zoledronic acid(ZOL)through intravenous injection and antibiotics in drinking water were administered for throughout the experiment.Pulp was exposed on the left maxillary first molar,then the mice were left for 5 weeks after which bilateral maxillary first molar was extracted and mice were left for additional 3 weeks to heal.All mice were harvested,and cecum,maxilla,and femurs were collected.ONJ development was assessed usingμCT and histologic analyses.When antibiotic was treated in mice,these mice had no weight changes,but developed significantly enlarged ceca compared to the control group(CTL mice).Periapical bone resorption prior to the tooth extraction was similarly prevented when treated with antibiotics,which was confirmed by decreased osteoclasts and inflammation.ZOL treatment with pulp exposure significantly increased bone necrosis as determined by empty lacunae and necrotic bone amount.Furthermore,antibiotics treatment could further exacerbate bone necrosis,with increased osteoclast number.Our findings suggest that the commensal microbiome may play protective role,rather than pathological role,in the early stages of MRONJ development.

关 键 词：protective finding ANTIBIOTICS 

分 类 号：R781.34[医药卫生—口腔医学]