糖蛋白96、Raf-1和Nei内切核酸酶Ⅷ样蛋白2表达与鼻咽癌患者放射治疗敏感性的相关性  

Correlation between the expressions of glycoprotein 96,Raf-1 and Nei endonucleaseⅧ-like 2 and the sensitivity to radiotherapy in patients with nasopharyngeal carcinoma

作　　者：訾滢洁 王彬[1] 高会霞 ZI Yingjie;WANG Bin;GAO Huixia(Department of Radiotherapy,Xuchang Central Hospital Affiliated to Henan University of Science and Technology,Xuchang 461000,Henan Province,China)

机构地区：[1]河南科技大学附属许昌市中心医院放射治疗科,河南许昌461000

出　　处：《新乡医学院学报》2022年第6期548-551,556,共5页Journal of Xinxiang Medical University

摘　　要：目的探讨糖蛋白96(gp96)、Raf-1及Nei内切核酸酶Ⅷ样蛋白2(NEIL2)在鼻咽癌(NPC)组织中的表达及其与放射治疗敏感性的相关性。方法选择2017年12月至2019年12月河南科技大学附属许昌市中心医院收治的125例疑似NPC患者为研究对象,根据病理组织学检查结果确诊为NPC 65例,鼻咽炎60例(对照组)。65例NPC患者确诊后均行放射治疗,放射治疗后根据治疗效果将患者分为放射治疗敏感组(n=48)和放射治疗抵抗组(n=17)。采用免疫组织化学法检测NPC组织和鼻咽炎患者鼻咽部黏膜组织中gp96、Raf-1及NEIL2蛋白的表达,采用Kendall分析gp96、Raf-1及NEIL2蛋白与NPC患者放射治疗敏感性的关系。结果放射治疗敏感组和放射治疗抵抗组患者NPC组织中gp96、Raf-1、NEIL2蛋白表达显著高于对照组,放射治疗抵抗组患者NPC组织中gp96、Raf-1、NEIL2蛋白表达显著高于放射治疗敏感组(P<0.05)。Kendall相关分析结果显示,gp96、Raf-1及NEIL2蛋白表达与NPC患者的放射治疗敏感性均呈显著负相关(r=-0.323、-0.618、-0.396,P<0.01)。结论NPC组织中gp96、Raf-1和NEIL2蛋白高表达,且gp96、Raf-1和NEIL2蛋白表达水平与NPC的放射治疗敏感性呈负相关,抑制gp96、Raf-1和NEIL2蛋白表达有望提高NPC的放射治疗敏感性。Objective To investigate the expressions of glycoprotein 96(gp96),Raf-1 and Nei endonucleaseⅧ-like 2(NEIL2)in nasopharyngeal carcinoma(NPC)tissues and their correlation with the sensitivity to radiotherapy.Methods A total of 125 patients with suspected NPC admitted to Xuchang Central Hospital Affiliated to Henan University of Science and Technology from December 2017 to December 2019 were selected as the research subjects.Among them,65 patients were diagnosed as NPC and 60 patients were diagnosed as nasopharyngitis(control group)according to the results of histopathological examination.All of the 65 NPC patients received radiotherapy after diagnosis.After radiotherapy,the patients were divided into radiotherapy sensitivity group(n=48)and radiotherapy resistance group(n=17)according to the treatment effect.The expressions of gp96,Raf-1 and NEIL2 proteins in NPC tissues and nasopharyngeal mucosa tissues of patients with nasopharyngitis were detected by immunohistochemistry.The relationship between gp96,Raf-1,NEIL2 proteins and the sensitivity to radiotherapy in NPC patients was analyzed by Kendall correlation.Results The expressions of gp96,Raf-1 and NEIL2 proteins in NPC tissues of patients in the radiotherapy sensitivity group and radiotherapy resistance group were significantly higher than those in the control group(P<0.05).The expressions of gp96,Raf-1 and NEIL2 proteins in NPC tissues of patients in the radiotherapy resistance group were significantly higher than those in the radiotherapy sensitivity group(P<0.05).Kendall correlation analysis results showed that the expressions of gp96,Raf-1 and NEIL2 proteins were significantly negatively correlated with the sensitivity of NPC patients to radiotherapy(r=-0.323,-0.618,-0.396;P<0.01).Conclusion The expressions of gp96,Raf-1 and NEIL2 proteins in NPC tissues are up-regulated,and the expression levels of gp96,Raf-1 and NEIL2 proteins are negatively correlated with the sensitivity of NPC to radiotherapy.Inhibiting the expressions of gp96,Raf-1 and NEIL2 proteins

关 键 词：鼻咽癌 糖蛋白96 RAF-1 Nei内切核酸酶Ⅷ样蛋白2 放射治疗 敏感性 

分 类 号：R739.63[医药卫生—肿瘤]