Co-immunolocalization of Disc1 and Gas7 protein in adult mice brain  

作　　者：Udaya Kumar 

机构地区：[1]Unit of Biochemistry,Department of Zoology,University of Madras,Chennai,Tamil Nadu,India [2]Department of Neurology,University of California Los Angeles,Los Angeles,California,U.S.A.

出　　处：《Brain Science Advances》2022年第1期70-77,共8页神经科学（英文）

摘　　要：Objective: The aim of the present study was to check the potential interaction of two neurodevelopmental proteins, Disc1 and Gas7, in the adult mice brain. Methods: Twenty-four male Swiss albino mice were used for the study. The mice were 12 weeks old in the beginning of the experiment. Immunohistochemistry and co-immunofluorescence were performed on the coronal sections of mice brain and immunoblotting and co-immunoprecipitation were done on the whole brain lysate. Results: Data from immunohistochemistry and co-immunofluorescence indicate the occurrence and co-localization of Disc1 and Gas7 proteins in soma and projections of the brain cells. Immunostaining was observed in cerebral cortex, hypothalamus, midbrain, pons, medulla oblongata and CA3 of hippocampus of the brain. The data from Immunoblotting and co-immunoprecipitation validates the presence and interaction of Disc1 and Gas7 protein in whole brain lysate. Conclusion: Data indicates the potential interaction of Disc1 and Gas7 protein in adult brain. The study highlights the need for further research on Disc1-Gas7 protein interaction in brain development and neuro-disorders.

关 键 词：Disc1 Gas7 co-immunolocalization co-immuno-fluorescence CO-IMMUNOPRECIPITATION 

分 类 号：R392[医药卫生—免疫学]