MicroRNA-146a对急性胰腺炎胰腺腺泡细胞增殖及凋亡的影响及其机制研究  

The effects and mechanisms of microRNA-146a in the proliferation and apoptosis of pancreatic acinar cells

作　　者：王宝友[1] 潘宏年[1] 王修中[1] 王凤 汪发勇[1] Bao-you Wang;Hong-nian Pan;Xiu-Zhong Wang;Feng Wang;Fa-yong Wang(Department of gastroenterology,Lu'an People's Hospital,Lu'an,Anhui,237005,China)

机构地区：[1]六安市人民医院消化内科,安徽六安237005

出　　处：《中国现代医学杂志》2022年第12期14-19,共6页China Journal of Modern Medicine

基　　金：安徽省自然科学基金(No:1808085MH293)。

摘　　要：目的探讨microRNA-146a(miR-146a)对急性胰腺炎胰腺腺泡细胞增殖及凋亡的影响,并分析相关机制。方法体外诱导并培养急性胰腺炎MPC-83细胞,将急性胰腺炎MPC-83细胞分为阴性对照组(mimics-NC组)、过表达miR-146a组(miR-146a-mimics组),另以未经诱导处理的MPC-83细胞为空白对照组(NG组)。采用实时荧光定量聚合酶链反应检测各组MPC-83细胞miR-146a,MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡情况,酶联免疫吸附试验检测肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6),Western blotting检测增殖细胞核抗原(PCNA)、Bcl-2相关X蛋白(Bax)、B淋巴细胞瘤2(Bcl-2)、核转录因子-κB p65(NF-кB p65)蛋白的表达。结果急性胰腺炎细胞模型建立成功,成功转染后,与NG组、mimicsNC组比较,miR-146a-mimics组细胞凋亡率,TNF-α、IL-6、Bax和NF-κB p65蛋白相对表达量降低(P<0.05),细胞存活率、PCNA和Bcl-2蛋白相对表达量升高(P<0.05)。结论过表达miRNA-146a可抑制急性胰腺炎MPC-83细胞凋亡并促进细胞增殖,其作用可能通过抑制NF-кB通路活化来实现。Objective To investigate the effects of microRNA-146a(miR-146a)on the proliferation and apoptosis of pancreatic acinar cells in acute pancreatitis,and to analyze the related mechanisms.Methods MPC-83cells were induced as acute pancreatitis models and cultured in vitro,and were divided into negative control group(mimics-NC group)and over-expression miR-146a-mimics group(miR-146a-mimics group).The uninduced MPC-83 cells were set as the blank control group(NG group).The level of miR-146a in MPC-83 cells was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The cell proliferation was detected by MTT assay,while the cell apoptosis was detected by flow cytometry.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6).The protein expressions of proliferating cell nuclear antigen(PCNA),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and nuclear factor kappa B(NF-κB)p65 were detected via Western blotting.Results Acute pancreatitis cell models were established successfully.After transfection,the cell apoptosis rate,the levels of TNF-αand IL-6,and the protein expressions of Bax and NF-κB p65 in the mi R-146a-mimics group were lower than those in the NG group and the mimics-NC group(P<0.05).In contrast,the cell viability and the relative protein expressions of PCNA and Bcl-2were increased in the miR-146a-mimics group compared with those in the NG group and the mimics-NC group(P<0.05).Conclusions Overexpression of miR-146a inhibits the apoptosis of acute pancreatitis MPC-83 cells but promotes their proliferation,possibly by suppressing the activation of NF-κB pathway.

关 键 词：急性胰腺炎 胰腺腺泡细胞 microRNA-146a 核因子-κB通路 增殖 凋亡 

分 类 号：R576[医药卫生—消化系统]