脐带间充质干细胞培养上清液对食管鳞癌细胞球的影响  

Effect of umbilical cord mesenchymal stem cell supernatant on esophageal squamous cell carcinoma spheres

作　　者：杜鸣 刘卓健 邹锋 蔡慧红 李婧逸 周博 张潇凡 耿静文 张燕松 马军[1,2] DU Ming;LIU Zhuojian;ZOU Feng;CAI Huihong;LI Jingyi;ZHOU Bo;ZHANG Xiaofan;GENG Jingwen;ZHANG Yansong;MA Jun(Medical Research Center,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China;Clinical Laboratory,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China)

机构地区：[1]郑州大学第二附属医院医学研究中心,河南郑州450014 [2]郑州大学第二附属医院检验科,河南郑州450014

出　　处：《胃肠病学和肝病学杂志》2022年第6期634-637,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：河南省科技厅重点研发与推广专项(科技攻关)项目(202102310104,212102310140,212102310048,22170021);河南省医学科技攻关项目省部共建计划(SBGJ202102176)。

摘　　要：目的探索脐带间充质干细胞(umbilical cord mesenchymal stem cells,UCMSCs)培养上清液对食管鳞癌(esophageal squamous cell carcinoma,ESCC)细胞系KYSE450成球的作用。方法收集UCMSCs培养上清液并制备冻干粉,在对KYSE450进行成球培养过程中加入含有不同浓度上清液冻干粉的类器官培养基(organoid medium,OM),利用高内涵成像系统观察各浓度组KYSE450的成球情况。结果KYSE450细胞接种24 h后(Day 1)有少量细胞聚集生长,随后各组细胞球直径逐渐增大;培养至第6天(Day 5),OM组、添加上清液冻干粉浓度30μg/ml组及3μg/ml组出现部分细胞球崩解;第7天(Day 6),3000μg/ml组及300μg/ml组细胞团仍能保持完整的细胞球形态,其他组均有大量细胞球崩解。结论UCMSCs上清液可以促进KYSE450成球。Objective To explore the effect of the supernatant of umbilical cord mesenchymal stem cells(UCMSCS)on the spheroidization of esophageal squamous cell carcinoma(ESCC)cell line KYSE450.Methods The culture supernatant of UCMSCs was collected and made into lyophilized powder;organoid medium(OM)containing different concentrations of supernatant lyophilized powder was added during the spherogenesis of KYSE450 and the spherogenesis of KYSE450 in each group was observed using high-inclusion imaging system.Results After 24 hours of cell seeding(Day 1),a small number of cells grew in aggregates,followed by a gradual increase in the diameter of cell spheres in each group;on the 6th day(Day 5),some cell spheres disintegrated in OM group,30μg/ml group and 3μg/ml group;on the 7th day(Day 6),the cell spheres in 3000μg/ml and 300μg/ml groups remained intact,while a large number of cell spheres disintegrated in all other groups.Conclusion UCMSCs supernatant can promote sphere-formation KYSE450.

关 键 词：脐带间充质干细胞 分泌蛋白 食管鳞癌 3D培养 

分 类 号：R735.1[医药卫生—肿瘤]