家兔血管外给药对乙酰氨基酚的药动学实验教学改革探究  

Reform of Pharmacokinetic Experimental Teaching on Parenteral Administrat ion of APAP in Rabbits

作　　者：王红芳 李春花 张璇 王捧英 刘时乔 李国川 Wang Hongfang;Li Chunhua;Zhang Xuan;Wang Pengying;Li Guochuan(School of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200;School of Basic Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200;Experiment Center,Hebei University of Chinese Medicine,Shijiazhuang 050200,China)

机构地区：[1]河北中医学院药学院,河北石家庄050200 [2]河北中医学院基础医学院,河北石家庄050200 [3]河北中医学院实验中心,河北石家庄050200

出　　处：《广东化工》2022年第11期230-232,共3页Guangdong Chemical Industry

基　　金：河北省研究生课程思政示范课程建设项目,YKCSZ2021079;河北中医学院教育教学改革研究项目,19yb-32。

摘　　要：家兔血药浓度法测定对乙酰氨基酚(Paracetamol,APAP)的药动学参数和生物利用度实验是生物药剂学相关课程的重要实验之一,该实验在学生掌握药动学参数及计算方法方面意义重大。为合理配置实验教学资源、提高实验质量,对实验内容和实验模式进行积极地探索性改革。改进的结果显示,采用高效液相色谱法测定APAP含量,可明显减少家兔取血量,简化了血样处理步骤,且检测结果的精确性和稳定性明显提高,同时,也极大提高了学生对该课程的学习主动性和科研兴趣。The pharmacokinetic parameters and bioavailability of paracetamol(APAP)in rabbit blood concentration assay is one of the important experiments in the course of biopharmaceutics.It plays an important role in students’understanding of pharmacokinetic parameters and calculation methods.In order to reasonably allocate experimental teaching resources and improve experimental quality,the experimental content and experimental mode was reformed actively.The improved results showed that the blood collection of rabbits was significantly reduced,the procedure of blood sample processing was simplified,and the accuracy and stability of the test results were obviously improved when the content of APAP was determined by HPLC.The experimental reform also greatly improved students’learning initiative and research interest in the course.

关 键 词：生物药剂学 药物动力学 教学改革 对乙酰氨基酚 HPLC 

分 类 号：R-4[医药卫生] G642.423[文化科学—高等教育学]