利用杏鲍菇残渣制备D-氨基葡萄糖盐酸盐的研究  

Study on the Preparation of D-Glucosamine Hydrochloride Using the Residue of Pleurotus eryngii

作　　者：崔玮 丁玲强[1,2] 李佩佩 李学艳 腾琪 李彩霞 李玉兰 CUI Wei;DING Ling-qiang;LI Pei-pei;LI Xue-yan;TENG Qi;LI Cai-xia;LI Yu-lan(College of Life Sciences and Engineering,Hexi University,Zhangye 734000,China;Laboratory Animal Center,Hexi University,Zhangye 734000,China;Middle School of Ganzhou,Zhangye 734000,China)

机构地区：[1]河西学院生命科学与工程学院,甘肃张掖734000 [2]河西学院实验动物中心,甘肃张掖734000 [3]甘肃省张掖市甘州中学,甘肃张掖734000

出　　处：《中国食用菌》2022年第6期70-74,81,共6页Edible Fungi of China

基　　金：甘肃省河西走廊特色资源利用重点实验室面上项目(XZ2001)。

摘　　要：试验探究了利用杏鲍菇残渣制备D-氨基葡萄糖盐酸盐的工艺。考察了料液比、反应时间、反应温度、盐酸浓度4个单因素变量对D-氨基葡萄糖盐酸盐得率的影响,在此基础上进行了L9(34)正交试验以确定最佳工艺,按照最佳工艺制备了D-氨基葡萄糖盐酸盐,并测定样品的氯离子含量和比旋光度,利用纸层析法和红外光谱对样品进行初步鉴定。结果显示,利用杏鲍菇残渣制备D-氨基葡萄糖盐酸盐的理想工艺为:反应时间3.5 h、料液比1∶6、反应温度100℃、盐酸浓度30%,D-氨基葡萄糖盐酸盐的得率可达5.23%;经测定样品中氯离子含量为18.14%,层析分析和红外光谱分析表明该方法制备的D-氨基葡萄糖盐酸盐的纯度较高。工艺的确定为利用杏鲍菇残渣制备D-氨基葡萄糖盐酸盐提供了参考。The preparation of D-glucosamine hydrochloride from Pleurotus eryngii residue was investigated.The effects of solid-liquid ratio,reaction time,reaction temperature and concentration of hydrochloric acid on the yield of D-glucosamine hydrochloride were investigated.On this basis,L9(34)orthogonal test was carried out to determine the optimal process.D-glucosamine hydrochloride was prepared according to the optimal process,and the chlorine ion content and specific rotation of the sample were determined.The samples were identified by paper chromatography and infrared spectroscopy.The results showed that the optimal preparation conditions of D-glucosamine hydrochloride from P.eryngii residue were as follows:reaction time 3.5 h,solid-liquid ratio 1:6,reaction temperature 100℃,hydrochloric acid concentration 30%,the yield of D-glucosamine hydrochloride could reach 5.23%.The content of chlorine ion in the sample was 18.14%.The chromatographic analysis and spectral analysis showed that the purity of D-glucosamine hydrochloride prepared by this method was high.This process provided reference for preparing D-glucosamine hydrochloride from P.eryngii residue.

关 键 词：杏鲍菇 残渣 D-氨基葡萄糖盐酸盐 正交试验 

分 类 号：S646.1[农业科学—蔬菜学]