间歇性缺氧小鼠血清外泌体对Lewis肺癌细胞上皮间充质转化及侵袭转移的影响  

Effects of serum exosomes in intermittent hypoxia mice on epithelial-mesenchymal transformation, invasion and metastasis of Lewis lung cancer cells

作　　者：成林杰 姚巧玲 CHENG Linjie;YAO Qiaoling(Department of Physiology,School of Basic Medical Sciences,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830011,China)

机构地区：[1]新疆医科大学基础医学院生理学教研室,新疆乌鲁木齐830011

出　　处：《中国医药导报》2022年第17期5-9,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81860022)。

摘　　要：目的 探讨间歇性缺氧(IH)小鼠血清外泌体对Lewis肺癌细胞(LLC)上皮间充质转化(EMT)及侵袭转移的影响。方法 选用80只C57BL/6J雄性小鼠,7~8周龄,体重22~25 g,将体重相近的两只小鼠随机分为室内空气组(RA)和IH组,两组小鼠平均体重相差≤2 g,每组40只。IH组进行IH刺激,每个循环1 min,氧分压从(6±1)%/30 s到(21±1)%/30 s,12 h/d,连续4周。RA组小鼠饲养于普通笼子。从小鼠血清中提取外泌体;CCK-8法检测细胞活力;Transwell实验和细胞划痕实验检测细胞的侵袭和迁移能力;Western blot检测E-钙黏蛋白、N-钙黏蛋白和基质金属蛋白酶9(MMP-9)蛋白表达水平。结果 小鼠血清外泌体孵育LLC细胞后,IH组的活细胞数高于RA组,差异有高度统计学意义(P <0.01);IH组细胞侵袭和迁移能力高于RA组,差异有高度统计学意义(P <0.01);IH组E-钙黏蛋白表达水平低于RA组,差异有统计学意义(P <0.05);IH组N-钙黏蛋白表达水平高于RA组,差异有统计学意义(P <0.05);两组MMP-9表达水平比较,差异无统计学意义(P> 0.05)。结论 IH小鼠血清外泌体可能诱导LLC细胞EMT及侵袭转移。Objective To investigate the effect of serum exosomes in intermittent hypoxia(IH) mice on epithelial-mesenchymal transition, invasion and metastasis of Lewis lung cancer cells(LLC). Methods Eighty C57BL/6J male mice,7-8 weeks old, weighing 22-25 g were selected. Two mice with similar body weight were randomly divided into indoor air group(RA) and IH group. The difference in average body weight of mice between the two groups was ≤2 g, with40 mice in each group. In the IH group, IH stimulation was performed for one minute per cycle, and the oxygen partial pressure was changed from(6±1)%/30 s to(21±1)%/30 s, 12 h/d, for four consecutive weeks. Mice in RA group were housed in ordinary cages. Extraction of exosomes from mouse serum;cell viability was detected by CCK-8 method;Transwell assay and cell scratch assay were used to detect cell invasion and migration ability;Western blot was used to detect the protein expression levels of E-cadherin, N-cadherin, and matrix metalloproteinase 9(MMP-9). Results After incubating LLC cells with mouse serum exosomes, the number of viable cells in the IH group was higher than that in the RA group, and the difference was highly statistically significant(P < 0.01);the ability of cell invasion and migration in the IH group was higher than that in the RA group, and the difference was highly statistically significant(P < 0.01);the expression level of E-cadherin in the IH group was lower than that in the RA group, and the difference was statistically significant(P < 0.05);the expression level of N-cadherin in the IH group was higher than that in the RA group,and the difference was statistically significant(P < 0.05);therei was no significant difference in the expression levels of MMP-9 between the two groups(P > 0.05). Conclusion Serum exosomes from IH mice may induce EMT and invasion and metastasis of LLC cells.

关 键 词：间歇性缺氧 LEWIS肺癌细胞 上皮间充质转化 转移 

分 类 号：R364.4[医药卫生—病理学]