不同夜间温度处理对马铃薯试管薯及块茎形成相关基因表达的影响  

The Effects of Different Night Temperature Treatments on in vitro Tuberization and Related-Genes Expression in Potato

作　　者：刘菊 李广存[1] 段绍光[1] 胡军[1] 简银巧 刘建刚 金黎平[1] 徐建飞[1] Liu Ju;Li Guangcun;Duan Shaoguang;Hu Jun;Jian Yinqiao;Liu Jiangang;Jin Liping;Xu Jianfei(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops,Ministry of Agriculture and Rural Affairs,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所/农业农村部薯类作物生物学和遗传育种重点实验室,北京100081

出　　处：《作物杂志》2022年第3期92-98,共7页Crops

基　　金：国家自然科学基金(31771860);国家马铃薯产业技术体系项目(CARS-9)。

摘　　要：为探究不同夜间温度处理对马铃薯试管薯形成的影响,以7个马铃薯品种或材料的试管苗为试材,在昼/夜温度分别为23℃/13℃、23℃/18℃和23℃/23℃条件下进行试管薯的诱导培养,调查记录试管薯初始结薯时间、结薯率和结薯方式变化,并分析不同夜间温度处理下试管薯初始形成植株及同期未形成试管薯植株参与块茎形成相关基因的表达量变化。结果表明,对于大多数基因型,与其他处理相比,23℃/13℃处理显著延长试管薯初始结薯时间,23℃/23℃处理降低结薯率,而23℃/18℃处理结薯较早且试管薯结薯率平均达84.3%,最利于试管薯形成。夜间温度可通过影响生物钟、糖代谢和光周期通路中StFKF1、StCO1和StSWEET1等相关基因的表达而调控块茎形成。In order to investigate the effects of different night-time temperature treatments on in vitro tuberization in potato,the initial time,percentage and type of in vitro tuberization were recorded using seven potato varieties or materials induced cultured at day/night temperature of 23℃/13℃,23℃/18℃and 23℃/23℃.The expression changes in tuberization-related genes were also analysed using initially microtuber formed and non-formed plants.The results showed that for most potato genotypes,compared with other treatments,23℃/13℃treatment significantly prolonged the initial time of in vitro tuberization,23℃/23℃treatment reduced the percentage of in vitro tuberization,23℃/18℃treatment tuberization time was early and the average percentage was 84.3%,the most conducive to the formation of test in vitro tuberization.Potato in vitro tuberization formation was affected on night temperature through regulating gene expression of circadian clock,sugar metabolism and photoperiodic signaling pathway,such as StFKF1,StCO1 and StSWEET1.

关 键 词：马铃薯 试管薯形成 夜间温度 初始结薯时间 基因表达 

分 类 号：S532[农业科学—作物学]