A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae  

作　　者：Vasil D’Ambrosio Lea G.Hansen Jie Zhang Emil D.Jensen Dushica Arsovska Marcos Laloux Tadas Jakociunas Pernille Hjort Davide De Lucrezia Serena Marletta Jay D.Keasling Michael K.Jensen 

机构地区：[1]Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Kgs.Lyngby,Denmark [2]Explora Biotech Srl,Italy [3]Joint BioEnergy Institute,Emeryville,CA,USA [4]Biological Systems and Engineering Division,Lawrence Berkeley National Laboratory,Berkeley,CA,USA [5]Department of Chemical and Biomolecular Engineering,Department of Bioengineering,University of California,Berkeley,CA,USA [6]Center for Synthetic Biochemistry,Institute for Synthetic Biology,Shenzhen Institutes of Advanced Technologies,Shenzhen,China

出　　处：《Synthetic and Systems Biotechnology》2022年第2期657-663,共7页合成和系统生物技术（英文）

基　　金：This study is supported by grants from the Novo Nordisk Foundation(NNF10CC1016517);Horizon 2020(MIAMi,814645);BioInnovation Institute Foundation(NNF19OC0055591 and NNF20SA0067054).

摘　　要：The synthetic biology toolkit for baker’s yeast,Saccharomyces cerevisiae,includes extensive genome engineering toolkits and parts repositories.However,with the increasing complexity of engineering tasks and versatile applications of this model eukaryote,there is a continued interest to expand and diversify the rational engineering capabilities in this chassis by FAIR(findable,accessible,interoperable,and reproducible)compliance.In this study,we designed and characterised 41 synthetic guide RNA sequences to expand the CRISPR-based genome engineering capabilities for easy and efficient replacement of genomically encoded elements.Moreover,we characterize in high temporal resolution 20 native promoters and 18 terminators using fluorescein and LUDOX CL-X as references for GFP expression and OD600 measurements,respectively.Additionally,all data and reported analysis is provided in a publicly accessible jupyter notebook providing a tool for researchers with low-coding skills to further explore the generated data as well as a template for researchers to write their own scripts.We expect the data,parts,and databases associated with this study to support a FAIR-compliant resource for further advancing the engineering of yeasts.

关 键 词：gRNA PROMOTER TERMINATOR YEAST STANDARDIZATION 

分 类 号：Q78[生物学—分子生物学]