LGR4、R-spondin2在胃癌组织中的表达及其预后相关性研究  

Expression of LGR4 and r-sondin2 in gastric cancer and its prognosis correlation analysis

作　　者：杨文斌[1] 杨钊[1] 周凯凯[1] YANGY Wen-bin;YANG Zhao;ZHOU Kai-kai(Department of Gastroenterology,Chenzhou First People's Hospital,Chenzhou 423000,China)

机构地区：[1]湖南省郴州市第一人民医院胃肠外科,湖南郴州423000

出　　处：《中国现代普通外科进展》2022年第6期439-443,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨富含亮氨酸重复序列的G蛋白偶联受体-4(LGR4)、R-spondin2在胃癌组织中的表达及其预后相关性。方法:2015年1月至2017年5月,151例胃癌患者术中癌灶组织151例,癌旁组织(距癌灶边缘≥5 cm)80例,采用免疫组织化学染色法检测组织中LGR4、R-spondin2表达,并分析其与胃癌患者临床病理特征的关系;采用乘积极限(K-M)法对胃癌患者进行生存分析,Log-rank检验比较显著性;采用COX回归模型分析胃癌患者预后影响因素。结果:与癌旁组织相比,癌灶组织LGR4、R-spondin2阳性表达率均较高(P<0.05)。胃癌组织中LGR4表达与性别、年龄均无关(P>0.05),与肿瘤直径、病理分级、临床分期、淋巴结转移均有关(P<0.05);R-spondin2表达与性别、年龄、肿瘤直径均无关(P>0.05),与病理分级、临床分期、淋巴结转移均有关(P<0.05)。151例胃癌患者在随访期内有72例死亡,病死率为47.68%,高于LGR4阴性表达者病死率(P<0.05),总生存期较短(P<0.05);R-spondin2阳性表达者病死率高于R-spondin2阴性表达者(P<0.05),总生存期较短(P<0.05)。COX多因素分析结果显示,LGR4阳性表达、R-spondin2阳性表达、低分化、Ⅲ~Ⅳ期、伴淋巴结转移均是影响胃癌患者预后的独立危险因素(P<0.05)。结论:胃癌组织中LGR4、R-spondin2均为高表达,且两者均与患者临床病理特征密切相关,是影响患者预后的独立危险因素,可能用于临床病情评估及预后死亡风险预测。Objective:To investigate the expression of leucine-rich domain-containing G protein-coupled receptor 4(LGR4) and R-spondin2 in gastric cancer and their prognostic significance.Methods:A total of 151 patients with gastric cancer admitted to our hospital from January 2015 to May 2017 were selected as the research objects.151 cases of tumor tissue and 80 cases of paracancerous tissue(more than 5 cm away from the edge of cancer focus) were collected during operation.Immunohistochemical staining was used to detect the expression of LGR4 and R-spondin2,the relationship between gastric cancer and clinicopathological features was analyzed;the survival of patients with gastric cancer was analyzed by product limit(K-M) method,Log-rank test was used to compare the significance;COX regression model was used to analyze the prognostic factors of gastric cancer patients.Results:The positive expression rates of LGR4 and R-spondin2 in tumor tissues were higher than those in adjacent tissues(P0.05),but correlated with tumor diameter,pathological grade,clinical stage and lymph node metastasis(P0.05),but correlated with pathological grade,clinical stage and lymph node metastasis(P<0.05).In the follow-up period,72 patients died,the mortality rate was 47.68%.Compared with LGR4 negative expression patients,LGR4 positive expression patients had higher mortality rate(P<0.05) and shorter overall survival time(P<0.05);compared with R-spondin2 negative expression patients,R-spondin2 positive expression patients had higher mortality rate(P<0.05) and shorter overall survival time(P<0.05).COX multivariate analysis showed that LGR4 positive expression,R-spondin2 positive expression,poor differentiation,stage Ⅲ~Ⅳ,and lymph node metastasis were independent risk factors for the prognosis of gastric cancer patients(P<0.05).Conclusion:Both LGR4 and R-spondin2 are highly expressed in gastric cancer,and they are closely related to the clinicopathological characteristics of patients.They are independent risk factors affecting the prognosis of patie

关 键 词：胃肿瘤 富含亮氨酸重复序列的G蛋白偶联受体-4 R-spondin2 预后 

分 类 号：R735.2[医药卫生—肿瘤]