非洲猪瘟病毒MGF360-10L的结构预测及亚细胞定位  

Structure prediction and subcellular localization of MGF360-10L of African swine fever virus

作　　者：彭江玲 易佳敏 朱国强 文圆 吴盼雪 李丹[1] 郑海学[1] PENG Jiang-ling;YI Jia-min;ZHU Guo-qiang;WEN Yuan;WU Pan-xue;LI Dan;ZHENG Hai-xue(National Foot-and-Mouth Disease Reference Laboratory/Staie Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agriculture Science,Lanzhou 730046,China)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医科学》2022年第5期537-546,共10页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2021YFD1800100);甘肃省科技厅重大专项(20ZD7NA006);家畜疫病病原生物学国家重点实验室自主课题(SKLVEB2020CGPY02);中国农业科学院科技创新工程重大科研计划项目(CAAS-ZDRW202006-03)。

摘　　要：为了解非洲猪瘟病毒(ASFV)多基因家族成员MGF360-10L基因及其编码蛋白的理化性质和结构特征,本研究在NCBI数据库中搜集到68株含有MGF360-10L序列的ASFV毒株,对这些序列进行比较并构建系统发育进化树;分析MGF360-10L基因及其编码蛋白的理化性质,预测蛋白二、三级结构;利用重组质粒验证在线网站预测的亚细胞定位结果。结果显示,MGF360-10L基因在Ⅱ型毒株中长度相同,基因同源性较高,与其他基因型毒株存在一定差异;理化性质分析结果显示,MGF360-10L蛋白不稳定系数为34.07,二级结构主要是ɑ-螺旋,说明该蛋白较稳定;在249~271 aa处存在跨膜区,不含核定位序列;间接免疫荧光试验结果也证实MGF360-10L蛋白定位在细胞质中。本研究探讨了MGF360-10L基因及其编码蛋白的结构特征,为了解其结构与功能间的关系提供了数据。In order to understand the physical and chemical properties and structural characteristicsof the MGF360-10L gene and its encoded protein,a member of the African swine fever virus(ASFV)multi-gene family,this study collected 68 ASFV strains containing MGF360-10L sequence in the NCBI database.These nucleotide sequences were compared and a phylogenetic tree was constructed;physical and chemical properties of the MGF360-10L encoded protein were analyzed;the secondary and tertiary structure of the protein were predicted;then recombinant plasmid was constructed to verify the subcellular localization which predicted by the online website.The results showed these lengths of MGF360-10L in genotypeⅡstrains were same with high homology,while certain differences existed between different genotype strains;the analysis of physical and chemical properties showed that the protein instability index of MGF360-10L was 34.07,with its secondary structure mainlyɑ-helix,indicating the protein is relatively stable;a transmembrane region was predicted at 249—271 amino acid while no nuclear localization sequence exist.The indirect immunofluorescence assay also confirmed that MGF360-10L protein is localized in the cytoplasm.This study explored the structural characteristics of MGF360-10L gene and its encoded protein,and provide datato understand its structure and function.

关 键 词：非洲猪瘟病毒 MGF360-10L基因 序列分析 蛋白结构预测 亚细胞定位 

分 类 号：S852.659.1[农业科学—基础兽医学]