氯胍通过下调FASN/SREBP-1c通路抑制肝癌细胞增殖、集落形成及迁移  

Proguanil inhibits liver cancer cells proliferation,colony formation,and migration by down-regulating the FASN/SREBP-1c pathway

作　　者：岳壮壮 黄玲俐 吴添雨 伍燕 颜新建 李高峰 YUE Zhuangzhuang;HUANG Lingli;WU Tianyu;WU Yan;YAN Xinjian;LI Gaofeng(Department of Oncology,Zhuzhou Hospital Affiliated to Xiangya School of Medicine,Central South University,Zhuzhou 412000,Hunan,China)

机构地区：[1]中南大学湘雅医学院附属株洲医院肿瘤科,湖南省株洲市412000

出　　处：《中南医学科学杂志》2022年第4期478-481,共4页Medical Science Journal of Central South China

基　　金：湖南省自然科学基金(2018JJ4097)。

摘　　要：目的探讨氯胍对肝癌细胞Hep-G2及SMMC-7721增殖、集落形成和迁移的影响及可能机制。方法用不同浓度的氯胍分别处理Hep-G2及SMMC-7721细胞。采用MTT法检测细胞增殖情况,克隆形成实验检测细胞集落形成能力,Transwell检测细胞迁移能力,Western blotting检测脂肪酸合成酶(FASN)及调节元件结合蛋白(SREBP-1c)的表达情况。结果氯胍抑制Hep-G2及SMMC-7721的增殖、集落形成及迁移能力;Western blotting显示氯胍下调FASN及SREBP-1c的表达。结论氯胍能抑制肝癌细胞Hep-G2及SMMC-7721增殖、集落形成及迁移,其作用机制可能与下调FASN/SREBP-1c通路有关。Aim To investigate the effects of Proguanil on proliferation,colony formation and migration of hepatocellular carcinoma cells Hep-G2 and SMMC-7721.Methods Hep-G2 and SMMC-7721 cells were treated with different concentrations of Proguanil.Cell proliferation was detected by MTT method,colony forming ability was detected by clone formation experiment,cell migration ability was detected by Transwell,and the expression of fatty acid synthase(FASN)and sterol regulatory element-binding protein-1c(SREBP-1c)was detected by Western blotting.Results Proguanil inhibited the proliferation,colony forming ability and migration of Hep-G2 and SMMC-7721;Western blotting showed that proguanil down-regulated the expression of FASN and SREBP-1c.Conclusion Proguanil can inhibit the proliferation,colony formation and migration of liver cancer cells Hep-G2 and SMMC-7721,and its mechanism may be related to down-regulation of the FASN/SREBP-1c pathway.

关 键 词：氯胍 肝细胞癌 增殖 FASN SREBP-1C 

分 类 号：R735.7[医药卫生—肿瘤]