A Long-wavelength Emissive Phenothiazine Derived Fluorescent Probe for Detecting HOCI Upregulation in 5-FU Stimulated Living Cells  

作　　者：WANG Bingya GUO Xiaomei LIU Zuodong WU Yongquan HOU Ji-Ting 

机构地区：[1]College of Chemistry and Chemical Engineering,Xinyang Normal University,Xinyang 464000,P.R.China [2]School of Chemistry and Chemical Engineering,Gannan Normal University,Ganzhou 341000,P.R.China [3]Henan Key Laboratory of Utilization of Non-metallic Mineral in the South of Henan,Xinyang Normal University,Xinyang 464000,P.R.China

出　　处：《Chemical Research in Chinese Universities》2022年第2期609-615,共7页高等学校化学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.21807029);the Double-Thousand Talents Plan of Jiangxi Province,China(2019);the Nanhu Scholars Program for Young Scholars of Xinyang Normal University,China.

摘　　要：The endogenous hypochlorous acid(HOCI) has been evidenced in a variety of cellular courses. However, the role of HOCI in most pathophysiological processes still keeps unclear because of the limited detecting tools. In this work, we presented the pre- paration of a phenothiazine-derived fluorescent probe CIO-1 for HOCI detection with a cyanopyridinium moiety to improve its water solubility and lengthen its emission wavelength. The HOCI-promoted oxidation of sulfur atom in the probe resulted in a 460-fold emission intensity enhancement at 635 nm with high selectivity and sensitivity(detection limit: 1.12 nmol/L). The rapid response(5 s) also endowed the probe with real-time detection ability. Successfully, CIO-1 was devoted to the bioimaging of endogenous HOCI in inflamed RAW 264.7 cells and 5-fluorouracil-treated MCF-7 cells.

关 键 词：Fluorescent probe PHENOTHIAZINE Hypochlorous acid 5-FLUOROURACIL BIOIMAGING 

分 类 号：O657.3[理学—分析化学]