基于双目视强度传输方程的多模式相位成像  

Multimodal phase imaging based on dual-view transfer of intensity equation

作　　者：陈超[1] 刘诚[1] CHEN Chao;LIU Chen(School of Science,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学理学院,江苏无锡214122

出　　处：《光学技术》2022年第3期277-285,共9页Optical Technique

基　　金：国家自然科学基金(61705092,U1730132)。

摘　　要：活细胞观测可以获得细胞的生命状态,是生物医学中众多研究的基础。然而多数活细胞因整体透明难以观测,通常使用泽尼克相衬及微分干涉相衬等相位成像技术来增强图像衬度。但通用的泽尼克相衬显微镜和微分干涉相衬显微镜仪器复杂、成本高昂,且依赖于沃拉斯顿棱镜和相衬环等特定光学元器件。提出了一种基于双目视强度传输方程的多模式相位成像方法,利用显微镜双目视筒上两个相机同步拍摄的物体欠焦及过焦图像,可求解强度传输方程得到物体的定量相位,并进一步计算物体的泽尼克相衬图像及微分干涉相衬图像。数值模拟和成像实验结果显示双目视多模式相位成像能够提供高质量的泽尼克相衬及微分干涉相衬图像。方法不需要借助特殊光学器件或者复杂光路,成本低且易于实现,有能力替代昂贵的相衬显微镜及微分干涉相衬显微镜,在非标记生物成像中获得更广泛的运用。Living cell observation reveals the life state of cells,which is the basis of many researches in biomedicine.However,most living cells are difficult to observe because of the transparency.Zernike Phase Contrast(ZPC)and Differential Interference Contrast(DIC)are usually used to enhance the image contrast.But the commonly used ZPC and DIC microscope are complex and expensive,and rely on Wollaston prism,phase contrast ring and other specific optical components.A multimodal phase imaging method based on dual-view transfer of intensity equation is proposed.With the defocus images acquired by two cameras installed in binocular tube of the microscope,the quantitative phase of the object can be obtained by solving the transfer of intensity equation,the ZPC image and the DIC image of the object could subsequently be computed.The results of numerical simulations and imaging experiments show that the dual-view multimodal phase imaging method can provides high quality ZPC and DIC image.The proposed method doesn’t rely on specific optical components or complex configuration,and is low-cost and easy to implement.Thus,it can substitute the expensive ZPC microscope and DIC microscope in the application of unlabeled biological imaging.

关 键 词：生物光学 相位成像 强度传输方程 泽尼克相衬 微分干涉相衬 多模式 非标记生物成像 

分 类 号：TN206[电子电信—物理电子学]