超抗原葡萄球菌肠毒素B对Lewis肺癌荷瘤小鼠抗肿瘤研究  

Anti-tumor effect of superantigen staphylococcal enterotoxin B gene for mice beared by Lewis lung cancer cell

作　　者：王小勇 冯敏 唐小军 WANG Xiaoyong;FENG Min;TANG Xiaojun(Department of Pediatric Surgery,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,P.R.China;Department of Cardiovascular Surgery,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,P.R.China;Department of Thoracic Surgery,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,P.R.China)

机构地区：[1]西南医科大学附属医院小儿外科,四川泸州646000 [2]西南医科大学附属医院心脏大血管外科,四川泸州646000 [3]西南医科大学附属医院胸外科,四川泸州646000

出　　处：《中国呼吸与危重监护杂志》2022年第3期195-199,共5页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的探讨超抗原葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)对Lewis肺癌细胞荷瘤小鼠肿瘤抑制作用。方法构建含SEB基因的表达质粒PCDH-SEB-GFP并转染Lewis肺癌细胞,采用蛋白免疫印迹法检测SEB表达;肿瘤细胞皮下注射方法构建Lewis荷瘤小鼠模型,成功后瘤内注射含有SEB表达质粒PCDH-SEB-GFP,观察其对小鼠体内Lewis肺癌生长的抑制作用。结果Lewis肺癌细胞转染SEB质粒PCDHSEB-GFP后可检测到SEB表达。瘤内注射质粒PCDH-SEB-GFP可显著抑制Lewis肺癌在荷瘤小鼠体内的生长。结论瘤内注射超抗原SEB表达质粒能抑制Lewis肺癌在小鼠体内的生长,以SEB作为外源性抗原基因的肺癌免疫基因治疗方法值得进一步研究。Objective To explore the tumor suppressive effect of superantigen staphylococcal enterotoxin B(SEB)for mice beared by Lewis lung cancer cell(LLCC).Methods SEB gene expressive plasmid PCDH-SEB-GFP was constructed and transfected into LLCC and it was detected by Western blot.Tumor-bearing mice model was established by subcutaneous injection of LLCC and SEB expressive plasmid PCDH-SEB-GFP was successfully injected into the tumor to observe its suppressive effect on the growth of Lewis lung cancer in mice.Results SEB expression was detected after transfection of LLCC with SEB gene plasmid PCDH-SEB-GFP.Intratumoral injection of plasmid PCDH-SEB-GFP could significantly inhibit the growth of Lewis lung cancer in tumor-bearing mice.Conclusions Intratumoral injection of superantigen SEB expressive plasmid can inhibit the growth of Lewis lung cancer in mice.It deserves a further study that SEB gene can work as an exogenous antigen gene in immune gene therapy for lung cancer.

关 键 词：肺癌 超抗原 葡萄球菌肠毒素B 免疫基因治疗 

分 类 号：R734.2[医药卫生—肿瘤]