Effect of Qinggan Huayu granule on H22 liver ascitic tumor mice  

作　　者：Fei-Ya Suo Xiao-Ran Zhu Zhen-Huan Yang Shu-Kun Yao 

机构地区：[1]Graduate School,Beijing University of Chinese Medicine,Beijing 100029,China [2]Department of Gastroenterology,China-Japanese Friendship Hospital,Beijing 100029,China

出　　处：《Journal of Hainan Medical University》2022年第9期1-7,共7页海南医学院学报（英文版）

基　　金：Fund Project:Beiing Municipal Science and Techoology Commission G20 Engineering Innovation Research for Ten Diseases Ten Drugs Research and Development Projec(N.Z171100001717008);National Key Research and Development Plan for Precision Medicine Research Key Phriject(No.2017YF0910002)。

摘　　要：Objective:To evaluate the therapeutic effect of Qinggan Huayu granule on mice with H22 liver cancer ascites tumor.Methods:A H22 liver cancer ascites mouse model was established by intraperitoneally injecting H22 liver cancer cells.Mice were randomly divided into the model group,the Ganfule group(1.35 g/kg),the fluorouracil group(50 mg/kg i.p),the Qinggan Huayu granule groups at low(0.67 g/kg),medium(1.34 g/kg),and high(2.68 g/kg)doses.Then the mice were administered continuously for 10 days and body weight and abdominal circumference were monitored every 3 days.On day 11,eight rats in each group were randomly selected for dissection to detect the amount of peritoneal water,peritoneal permeability and histopathological changes.The remaining mice were observed for survival.In addition,the vascular endothelial growth factor A(VEGFA)and vascular endothelial growth factor receptor 2(VEGFR2)were determined by Western blotting.Results:Compared with the model group,the weight growth of mice in the fluorouracil group and the medium-dose and high-dose Qinggan Huayu granule groups was slower(P<0.05).Moreover,the abdominal circumference of mice in each treatment group was increased slowly.There were significant differences in abdominal circumference between the fluorouracil group,the medium-dose group and the control group from day 6(P<0.05)while the abdominal circumference of the high dose group was significantly smaller than that of the control group from day 12(P<0.05).Moreover,compared with the model group,the amount of ascites in the medium-and high-dose Qinggan Huayu granule groups was decreased significantly(P<0.05).The optical density value of ascites supernatant in medium-and high-dose Qinggan Huayu granule group and the fluorouracil group decreased significantly(P<0.05)and the survival period of the medium-dose Qinggan Huayu granule group and the fluorouracil group was prolonged prominently(P<0.05).There was no significant difference in the low-dose Qinggan Huayu granule group and the Ganfule group.Peritoneal histo

关 键 词：H22 liver cancer ascites tumor Mouse Qinggan Huayu granule Survival period Peritoneal vascular permeability 

分 类 号：R73[医药卫生—肿瘤]