氨肽酶P2在上皮性卵巢癌组织中的表达及临床意义  

Expression and clinicopathological significance of aminopeptidase P2 in the epithelial ovarian cancer

作　　者：苏引利 李琳琳 莫佳娅 段浩然 张嘉琳 王秋杰 秦利影 吴晗[1] 谢娅[1] SU Yinli;LI Linlin;MO Jiaya;DUAN Haoran;ZHANG Jialin;WANG Qiujie;QIN Liying;WU Han;XIE Ya(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Oncology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Academy of Medical Sciences of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院妇产科,河南郑州450052 [2]郑州大学第一附属医院肿瘤科,河南郑州450052 [3]郑州大学医学科学院,河南郑州450052

出　　处：《肿瘤基础与临床》2022年第2期99-105,共7页journal of basic and clinical oncology

基　　金：国家自然科学基金青年基金项目(81802770);河南省医学科技攻关计划(联合共建)项目(2018020056);河南省科技攻关计划(重点)项目(192102310070)。

摘　　要：目的探讨氨肽酶P2(XPNPEP2)表达与上皮性卵巢癌临床病理特征和预后的关系。方法收集2019年1月至2021年9月郑州大学第一附属医院收治的15例非癌患者正常卵巢组织、40例上皮性卵巢癌患者原发灶和与之配对的35例转移癌灶和癌旁组织。采用免疫组化法及蛋白印迹法检测组织中XPNPEP2的表达;分析其与上皮性卵巢癌患者临床病理特征的关系。使用基因表达谱动态分析(GEPIA)数据库分析XPNPEP2表达与卵巢癌患者生存预后的关系。结果上皮性卵巢癌原发灶中XPNPEP2高表达率为82.50%(33/40),正常卵巢组织为13.33%(2/15)(χ^(2)=22.553,P<0.001)。转移癌灶中XPNPEP2高表达率为91.43%(32/35),癌旁组织高表达率为14.29%(5/35)(χ^(2)=41.794,P<0.001)。上皮性卵巢癌患者XPNPEP2表达水平与患者年龄、病理类型、分化程度、肿瘤大小、腹水以及FIGO分期均无相关性(χ^(2)=0.519,P=0.471;P=0.204;χ^(2)=0.275,P=0.600;χ^(2)=0.132,P=0.716;χ^(2)=0.519,P=0.471;χ^(2)=3.680,P=0.055)。卵巢癌中XPNPEP2的表达与患者总生存、疾病无进展生存无关(P=0.900、0.099)。结论XPNPEP2蛋白在上皮性卵巢癌原发灶及转移癌灶中表达上调,可作为上皮性卵巢癌精准治疗的新靶标。Objective To investigate the relationship between aminopeptidase P2(XPNPEP2)expression and clinicopathological features of epithelial ovarian cancer.Methods Surgical specimens were collected in the First Affiliated Hospital of Zhengzhou University from January 2019 to September 2021,including 15 cases of normal ovarian tissue,40 cases of the primary lesion of epithelial ovarian cancer and paired with 35 cases of metastatic cancer focal and para-cancerous ovarian tissue.Immunohistochemistry and Western Blotting were used to detect XPNPEP2 expression in tissue,and its relationship with clinicopathological features of patients with epithelial ovarian cancer was analyzed.Gene Expression Profiling Interactive Analysis(GEPIA)database was used to analyze the relationship between XPNPEP2 expression and survival and prognosis of patients with ovarian cancer.Results The high expression rate of XPNPEP2 in the primary lesion of epithelial ovarian cancer was 82.50%(33/40),and that in normal ovarian tissue was 13.33%(2/15)(χ^(2)=22.553,P<0.001).The high expression rate of XPNPEP2 in metastatic cancer focal was 91.43%(32/35),and that in para-cancerous tissue was 14.29%(5/35)(χ^(2)=41.794,P<0.001).The expression of XPNPEP2 was no correlated with the age,pathological type,degree of differentiation,tumor size,ascites and FIGO stage of epithelial ovarian cancer patients(χ^(2)=0.519,P=0.471;P=0.204;χ^(2)=0.275,P=0.600;χ^(2)=0.132,P=0.716;χ^(2)=0.519,P=0.471;χ^(2)=3.680,P=0.055).In addition,the high expression of XPNPEP2 in the primary lesion of epithelial ovarian cancer had no relation with the overall survival and disease progression free survival in the ovarian cancer patients(P=0.900,0.099).Conclusion XPNPEP2 protein is up-regulated in the primary lesion of epithelial ovarian cancer and metastatic cancer,which may serve as a novel target for precision therapy of epithelial ovarian cancer.

关 键 词：氨肽酶P2 上皮性卵巢癌 肿瘤转移 精准治疗 

分 类 号：R737.31[医药卫生—肿瘤]