HPVm16E7刺激树突状细胞SOCS1基因沉默后对宫颈癌小鼠模型免疫效应的影响  

Effect of HPVm16E7 Stimulated Dendritic Cell SOCS1 Gene Silencing on Immune Effect of Cervical Cancer Mouse Model

作　　者：左斌生 刘媛瑞 徐建华 罗宇虹 张战锋[3] ZUO Bin-sheng;LIU Yuan-rui;XU Jian-hua;LUO Yu-hong;ZHANG Zhan-feng(Laboratory Medicine,Nanfang Hospital of Southern Medical University,Guangzhou,Guangdong,510515,China;Laboratory of Tumor and Molecular Biology,Shunde Hospital of Guangzhou University of Traditional Chinese Medicine,Foshan,Guangdong,510006,China;Clinical Laboratory,The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong,510405,China)

机构地区：[1]南方医科大学南方医院检验医学科,广东广州510515 [2]广州中医药大学顺德医院肿瘤与分子生物学实验室,广东佛山510006 [3]广州中医药大学第一附属医院检验科,广东广州510405

出　　处：《现代生物医学进展》2022年第11期2013-2017,共5页Progress in Modern Biomedicine

基　　金：广东省中医药局科研项目(20222053);广东省教育厅基础研究重大项目(2017KZDXM019);创新强院青年科研人才培优项目(2015QN08)。

摘　　要：目的:在宫颈癌细胞TC-1的荷瘤小鼠模型中探讨树突状细胞(DCs)的细胞因子信号抑制物1(SOCS1)基因沉默后对宫颈癌细胞免疫效应的影响。方法:构建宫颈癌细胞系TC-1的荷瘤小鼠模型,将含有SOCS1沉默基因的慢病毒载体感染DCs细胞,对荷瘤小鼠进行细胞免疫后监测小鼠体内肿瘤生长、小鼠存活率,并检测小鼠脾细胞对TC-1细胞的体外裂解率以及γ干扰素(IFN-γ)、白细胞介素-12(IL-12)表达等指标。结果:DCs SOCS1基因沉默后可延长小鼠存活期,增强DCs对小鼠体内肿瘤生长的抑制作用,增强小鼠脾细胞对TC-1细胞的体外裂解率(P<0.05),增加小鼠血清IL-12因子表达(P<0.05)和小鼠脾细胞IFN-γ表达(P<0.05),但对小鼠脾内效应细胞的数量没有影响(P>0.05)。结论:小鼠体内实验初步证实,DCs SOCS1基因沉默后可一定程度上增强小鼠体内效应细胞对肿瘤细胞的杀伤效果。Objective:To investigate the effect of suppressor of cytokine signaling(SOCS1)gene silencing of dendritic cells(DCs)on the immune effect of cervical cancer cells in the tumor bearing mouse model of cervical cancer cell TC-1.Methods:The tumor bearing mouse model of cervical cancer cell line TC-1 was constructed.The lentivirus vector containing SOCS1 silencing gene was infected with DCS cells.After cellular immunization of tumor bearing mouse,the tumor growth and survival rate of mouse were monitored,the cleavage rate of spleen cells to TC-1 cells in vitro and the expression ofγinterferon(IFN-γ)and interleukin-12(IL-12)were detected.Results:DCs SOCS1 gene silencing can prolonged the survival of mouse,enhanced the inhibitory effect of DCs on tumor growth in vivo,enhanced the lysis rate of mouse spleen cells to TC-1 cells in vitro(P<0.05),increased the expression of IL-12 factor in serum of mouse(P<0.05)and IFN-γexpression of mouse spleen cells(P<0.05).However,the number of effector cells in spleen of mouse was not affected(P>0.05).Conclusion:In vivo experiments in mouse show that DCs SOCS1 gene silencing could enhance the killing effect of effector cells on tumor cells in mouse to a certain extent.

关 键 词：宫颈癌 树突状细胞 SOCS1 免疫治疗 

分 类 号：R-33[医药卫生] R737.33