纯钛表面微纳米形貌对MC3T3-E1细胞的调控作用及机制研究  

Regulatory Effect and Mechanism of Titanium Surface Micro-nano Topography on Adhesion,Migration and Osteogenic Differentiation of MC3T3-E1 Cells

作　　者：吴运 吴群 石梦琪 WU Yun;WU Qun;SHI Mengqi(Department of Stomatology,Naval Medical Center of PLA,Shanghai 200050,China)

机构地区：[1]中国人民解放军海军特色医学中心口腔科,上海200050

出　　处：《中国美容医学》2022年第6期78-83,共6页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金青年科学基金项目(名称:微纳米形貌通过影响细胞的机械记忆调控细胞成骨分化的研究,编号:31800804)。

摘　　要：目的:研究纯钛表面微纳米形貌对成骨前体细胞MC3T3-E1黏附、迁移及成骨分化的调控作用及机制。方法:纯钛表面抛光作为对照、接种MC3T3-E1细胞作为对照组;采用酸蚀+20 V电压阳极氧化的方法在纯钛表面制备微纳米形貌、接种MC3T3-E1细胞作为微纳米形貌组,转染阴性对照(NC)-siRNA或整合素α2-siRNA作为微纳米形貌+si-NC组、微纳米形貌+si-整合素α2组,给予二甲基亚砜(DMSO)或细胞外调节蛋白激酶(ERK)抑制剂PD98058作为微纳米形貌+DMSO组、微纳米形貌+PD98059组,检测黏附数目、迁移率、成骨诱导分化后OD_(540)及整合素α2、p-ERK、Runx2的表达水平。结果:电镜下观察,酸蚀+20 V电压阳极氧化制备得到管径100 nm的微纳米形貌。微纳米形貌组的黏附数目、迁移率、OD_(540)及整合素α2、p-ERK、Runx2的表达水平均高于对照组,差异有统计学意义(P<0.05)。微纳米形貌+si-整合素α2组的黏附数目、迁移率、OD_(540)及整合素α2、p-ERK、Runx2的表达水平均低于微纳米形貌+si-NC组,差异有统计学意义(P<0.05)。微纳米形貌+PD98059组的黏附数目、迁移率、OD_(540)及p-ERK、Runx2的表达水平均低于微纳米形貌+DMSO组,差异有统计学意义(P<0.05)。结论:纯钛表面微纳米形貌显著促进MC3T3-E1细胞黏附、迁移及成骨分化,这一促进作用与激活整合素α2/ERK/Runx2通路有关。Objective To investigate the effect and mechanism of titanium surface micro-nano topography on the adhesion,migration and osteogenic differentiation of MC3T3-E1 cells.Methods The pure titanium surface was polished for control,and MC3T3-E1 cells were inoculated as the control group.The micro-nano topography on the pure titanium surface was prepared by acid etching+20 V voltage anodization method,and MC3T3-E1 cells were inoculated as the micro-nano topography group.The negative control(NC)-siRNA or integrinα2-siRNA was transfected as the micro-nano topography+si-NC group,micro-nano topography+si-integrinα2 group.And DMSO or PD98058 was given as micro-nano topography+DMSO group,micro-nano topography+PD98059 group.The adhesion number,migration rate,OD_(540) after osteogenic differentiation and the expression level of integrinα2,p-ERK,Runx2 were detected.Results Under the electron microscope,the micro-nano topography with diameter of 100 nm was prepared by acid etching+20 V anodizing.The adhesion number,migration rate,OD_(540) and the expression levels of integrinα2,p-ERK and Runx2 in the micro-nano topography group were higher than those in the control group,the differences were statistically signi?cant(P<0.05).The adhesion number,migration rate,OD_(540) and the expression levels of integrinα2,p-ERK and Runx2 in the micro-nano topography+si-integrinα2 group were lower than those in the micro-nano topography+siNC group(P<0.05).The adhesion number,migration rate,OD_(540) and the expression levels of p-ERK and Runx2 in the micro-nano topography+PD98059 group were lower than those in the micro-nano topography+DMSO group(P<0.05).Conclusion The titanium surface micro-nano topography signi?cantly promote the adhesion,migration and osteogenic differentiation of MC3T3-E1 cells,which is related to the activation of integrinα2/ERK/Runx2 pathway.

关 键 词：微纳米形貌 纯钛表面改性 整合素Α2 黏附 迁移 成骨分化 信号通路 

分 类 号：R783.1[医药卫生—口腔医学]