Characterization,heterologous expression and engineering of trehalase for biotechnological applications  

作　　者：Han Gao Jin-Song Gong Chang Su Heng Li Zheng-Hong Xu Jin-Song Shi 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Life Sciences and Health Engineering,Jiangnan University,Lihu Avenue No.1800,Wuxi 214122,People’s Republic of China [2]National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,School of Biotechnology,Jiangnan University,Wuxi 214122,People’s Republic of China [3]Jiangsu Provincial Engineering Research Center for Bioactive Product Processing,Jiangnan University,Wuxi 214122,People’s Republic of China

出　　处：《Systems Microbiology and Biomanufacturing》2022年第3期445-460,共16页系统微生物学与生物制造（英文）

基　　金：supported by the National Key Research and Development Program of China(No.2021YFC2102000);the National Natural Science Foundation of China(No.21978116 and 32171261);the Ningxia Hui Autonomous Region Key Research&Development Plan(No.2019BCH01002).

摘　　要：Trehalose is a non-reducing disaccharide connected byα-1,1-glycosidic bonds;it is widely distributed in bacteria,fungi,yeast,insects,and plant tissues and plays various roles.It can be hydrolyzed by trehalase into two glucose molecules.Trehalases from different sources have been expressed in Escherichia coli,Pichia pastoris,Saccharomyces cerevisiae,baculovirus-silkworm,and other expression systems;however,it is most common in E.coli.The structural characteristics of different glycoside hydrolase(GH)family trehalases and the sources of trehalase have been analyzed.The catalytic mechanism of GH37 trehalase has also been elucidated in detail.Moreover,the molecular modification of trehalase has mainly focused on directed evolution for improving enzyme activity.We comprehensively reviewed the current application status and adaptable transformations was comprehensively overviewed in the context of industrial performance.We suggest that the level of recombinant production is far from meeting industrial requirements,and the catalytic performance of trehalase needs to be improved urgently.Finally,we discuss developmental prospects and future trends.

关 键 词：TREHALASE STRUCTURE EXPRESSION Catalytic mechanism APPLICATION 

分 类 号：O62[理学—有机化学]