2001—2018年上海市麻疹病毒流行株血凝素基因特征分析  

Genetic characteristics analysis of the hemagglutinin gene of measles virus in Shanghai,2001-2018

作　　者：杨玉颖[1] 陈达瑜 唐伟[1] 李云逸[1] 王嘉瑜[1] 李淑佩 李崇山 YANG Yuying;CHEN Dayu;TANG Wei;LI Yunyi;WANG Jiayu;LI Shupei;LI Chongshan(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Shanghai Institute of Infectious Disease and Biosecurity,Shanghai 200032,China)

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]上海交通大学医学院,上海200025 [3]上海重大传染病和生物安全研究院,上海200032

出　　处：《上海预防医学》2022年第5期436-440,共5页Shanghai Journal of Preventive Medicine

基　　金：上海市卫生健康委员会面上项目(201940311);上海市第五轮公共卫生三年行动计划(GWV-2);上海市科学技术委员会上海市青年科技英才扬帆计划(19YF1441500)。

摘　　要：【目的】分析2001—2018年上海市麻疹病毒(MeV)流行株血凝素(H)基因特征。【方法】收集2001—2018年上海市报告疑似麻疹病例的咽拭子样本,采用非洲绿猴肾细胞(Vero)/转染人信号淋巴细胞激活因子(hSLAM)细胞分离MeV,提取病毒核酸,采用逆转录聚合酶链反应(RT-PCR)扩增H基因全长,对扩增后的产物进行测序及序列分析,分析其基因特征。【结果】2001—2018年上海市麻疹风疹监测网络共收集咽拭子样本5665份,分离到1394株MeV,349株代表性MeV分离株H基因核苷酸和氨基酸同源性分别为87.4%~100.0%和85.1%~100.0%;与中国疫苗株(S191)核苷酸和氨基酸同源性为85.7%~100.0%和84.1%~100.0%。所有H1a基因亚型MeV分离株均丢失aa240所在的糖基化位点。【结论】2001—2018年上海市MeV流行株根据H基因进行的分子分型结果与N基因C末端450片段分子分型结果一致,绝大多数为H1a基因亚型,H蛋白氨基酸序列在主要功能位点上是比较保守的。[Objective]To analyze the genetic characteristics of the hemagglutinin(H)gene of measles virus(MeV)in Shanghai,2001-2018.[Methods]Nasopharyngeal swab specimens were collected from suspected measles cases reported in Shanghai from 2001 to 2018,and the isolation of measles virus was conducted with Vero/hSLAM cell line.RT-PCR amplification and sequencing were conducted after RNA extraction to analyze the genetic characteristics of the complete H gene.[Results]In total,5665 nasopharyngeal swab samples were collected by suspected measles case surveillance from 2001 to 2018,and 1394 measles virus strains were isolated.The homology of nucleotide acid and amino acid among 349 representative measles virus isolates was 87.4%-100.0%and 85.1%-100.0%,respectively.The homology of nucleotide acid and amino acid between representative measles virus isolates and China vaccine strain(S191)was 85.7%-100.0%and 84.1%-100.0%,respectively.All the sub-genotype H1a MeV isolates had an amino acid substitution(Ser240Asn),which removed a predicted N-linked glycosylation site.[Conclusion]Most of the MeV isolates are sub-genotype H1a analyzed based on H gene,which are identical to those of the N gene.The predicted amino acid sequences of the H protein are relatively conserved at most of the functionally significant amino acid positions.

关 键 词：麻疹病毒 血凝素 亲缘性分析 氨基酸变异 

分 类 号：R211[医药卫生—中医学] R511.1